Plasma concentration of apolipoprotein E in intermediate-sized remnant-like lipoproteins in normolipidemic and hyperlipidemic subjects
| Autor: | Madeleine Roy, Daniel Bouthillier, Jeffrey S. Cohn, Jean Davignon, Jacques Genest, Michel Tremblay, Mireille Amiot |
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| Rok vydání: | 1996 |
| Předmět: |
Apolipoprotein E
Adult Male medicine.medical_specialty Very low-density lipoprotein Apolipoprotein B Lipoproteins Hyperlipidemias Hyperlipoproteinemia Type IV Pathogenesis Hyperlipoproteinemia Type II chemistry.chemical_compound Apolipoproteins E Internal medicine Hyperlipoproteinemia Type III medicine Humans Chromatography High Pressure Liquid Triglycerides Electrophoresis Agar Gel biology Triglyceride Chemistry Cholesterol Fast protein liquid chromatography Middle Aged Lipoproteins LDL Endocrinology biology.protein lipids (amino acids peptides and proteins) Female Cardiology and Cardiovascular Medicine Lipoproteins HDL Lipoprotein |
| Zdroj: | Arteriosclerosis, thrombosis, and vascular biology. 16(1) |
| ISSN: | 1079-5642 |
| Popis: | Abstract Triglyceride-rich lipoprotein (TRL) remnants have been strongly implicated in the pathogenesis of atherosclerosis. To further investigate plasma remnant lipoprotein metabolism, we have determined the plasma concentration of apolipoprotein (apo) E (by polyclonal enzyme-linked immunoassay) in remnant-like lipoproteins, isolated by automated gel filtration chromatography as a fraction intermediate in size between VLDL and HDL. In normolipidemic subjects (n=12), 1.2±0.1 mg/dL (33±2%, mean±SE) of total plasma apoE was associated with this fraction (termed ISL apoE). In hypercholesterolemic (type IIa, n=12), hypertriglyceridemic (type IV, n=12), and mixed hyperlipidemic (type IIb, n=12) subjects, mean ISL apoE concentrations were 2.1±0.2, 2.5±0.2, and 3.8±0.4 mg/dL, respectively ( P r =.41, P P P r =.66), TRL cholesterol ( r =.49), TRL apoE ( r =.47), LDL apoB ( r =.42), and LDL+HDL triglyceride ( r =.74). These results suggest that (1) a significant proportion of plasma apoE resides within an intermediate-sized remnant-like lipoprotein fraction in both normolipidemic and hyperlipidemic subjects; (2) plasma remnant lipoprotein accumulation is associated with an elevation in ISL apoE concentration; and (3) ISL apoE concentration is significantly correlated with various proatherogenic lipid parameters and may itself be a potentially important atherogenic index. |
| Databáze: | OpenAIRE |
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