Vitamin E reduces endosulfan-induced toxic effects on morphology and behavior in early development of zebrafish (Danio rerio)
| Autor: | Samuel Penglase, K.L. Thorstensen, K. Dale, Ståle Ellingsen, J.D. Rasinger |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Insecticides medicine.medical_specialty Embryo Nonmammalian animal structures medicine.medical_treatment Developmental toxicity Danio Biology Toxicology medicine.disease_cause Antioxidants 03 medical and health sciences Internal medicine Gene expression medicine Animals Vitamin E Zebrafish Behavior Animal Neurotoxicity Embryo General Medicine biology.organism_classification medicine.disease Oxidative Stress 030104 developmental biology Endocrinology Endosulfan Oxidative stress Food Science |
| Zdroj: | Food and Chemical Toxicology. 101:84-93 |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/j.fct.2017.01.004 |
| Popis: | The aim of this study was to investigate if vitamin E (α-TOC) modulates the developmental toxicity of the pesticide endosulfan (ESF), using a modified zebrafish embryotoxicity test (ZET). Zebrafish (Danio rerio) embryos were exposed from 6 to 72 h post fertilization (hpf) to either ESF (0.1-50 mg/L) or α-TOC (0.01-3 mM) alone or in combination. The effects of these exposures on embryonic morphology, larval behavior and antioxidant gene expression were analyzed. Phenotypic analysis at 48 hpf showed that ESF led to a dose-dependent increase in embryonic deformities, including axis malformations, pericardial edema and reduced pigmentation. Co-exposure of ESF with α-TOC (1-3 mM) significantly (p 0.05) reduced ESF-induced embryonic malformations. Exposure to solely α-TOC did not affect rates of survival or malformations. Behavior studies showed that ESF caused hyperactivity at 5 days post fertilization, indicating a developmental neurotoxic effect. The ESF-induced hyperactivity was ameliorated by α-TOC. Elevated ESF concentrations caused down-regulation of the antioxidant genes cuzn-sod, gpx1a and cat, suggesting that ESF promoted oxidative stress in the embryos. α-TOC did not prevent the ESF-induced dysregulation of these genes. These results demonstrate that α-TOC protects against phenotypic and behavioral effects caused by ESF but did not rescue ESF-induced aberrations in antioxidant gene expression. |
| Databáze: | OpenAIRE |
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