Molecular identification of Trypanosoma brucei brucei and in vitro anti-trypanosomal activity of different parts of methanolic extract of Senna occidentalis
| Autor: | Ahmed Babangida Suleiman, Zainab Aliyu Alhafiz, Mohammed Sani Abdulsalami, Mohammed Auwal Ibrahim, T. Bulus |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
030231 tropical medicine
Trypanosoma brucei brucei lcsh:Medicine lcsh:RX1-681 Trypanosoma brucei 030226 pharmacology & pharmacy Senna occidentalis 03 medical and health sciences 0302 clinical medicine lcsh:Homeopathy parasitic diseases Ic50 values General Environmental Science Molecular identification Stem bark Anti-trypanosomal activity biology Chemistry lcsh:R Ribosomal RNA biology.organism_classification In vitro Polymerase chain reaction Biochemistry Gas chromatography-mass spectrometry General Earth and Planetary Sciences |
| Zdroj: | Clinical Phytoscience, Vol 6, Iss 1, Pp 1-7 (2020) |
| ISSN: | 2199-1197 |
| DOI: | 10.1186/s40816-020-00178-9 |
| Popis: | Objective The present study investigated the anti-Trypanosoma brucei brucei activity of methanolic extract of Senna occidentalis roots, leaves, stem bark, and seed in vitro and bioactive components of the most active plant part. Materials and methods Trypanosoma brucei brucei was identified by PCR, cultured in Dulbecco’s Modified Eagle Medium (DMEM) and incubated at 37 °C and 5% CO2. Subsequently, the cultured T. brucei brucei were incubated with 500 μg/ml concentration of different parts of S. occidentalis plant and the most potent fraction was identified and subjected to Gas Chromatography-Mass Spectrometry (GC-MS). Results Amplicons of the rRNA gene of T. brucei brucei detected had a size of 1200 bp. The anti-trypanosomal activities indicated that the root extract of the plant was the most active at 500 μg/ml with inhibitory activity of 88.89% and fractions (2 and 5) the most active with IC50 values of 1.49 and 1.16 μg/ml respectively. Conclusion Results from this study insinuates that phenolic and simple aliphatic compounds might play key role in the anti-trypanosomal activity of S. occidentalis roots. |
| Databáze: | OpenAIRE |
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