The rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets
Autor: | Jeremy A. Roberts, Erik H. Murchie, Kevin A. Pyke, Rita S Borna, Zinnia H. González-Carranza |
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Jazyk: | angličtina |
Rok vydání: | 2022 |
Předmět: |
Prostaglandin E receptor 3
microRNA pathway Ubiquitin-Protein Ligases Arabidopsis Plant Science Flowers Biology medicine.disease_cause F-box protein Gene Expression Regulation Plant Pollen microRNA flower development medicine Gene Research Articles F-box proteins Panicle Plant Proteins Oryza sativa food and beverages Oryza biology.organism_classification Cell biology MicroRNAs biology.protein lipids (amino acids peptides and proteins) Rice F‐box proteins Agronomy and Crop Science micro RNA pathway Biotechnology Research Article |
Zdroj: | Plant Biotechnology Journal |
ISSN: | 1467-7644 1467-7652 |
Popis: | Summary ERECTA PANICLE 3 (EP3) and ORYZA SATIVA F‐BOX KELCH 1 (OsFBK1) proteins share 57% and 54% sequence identity with the Arabidopsis F‐box protein HAWAIIAN SKIRT (HWS). Previously we showed that EP3 is a functional orthologue of HWS. Here we demonstrate that OsFBK1 is another functional orthologue of HWS and show the complexity of interaction between EP3 and OsFBK1 genes at different developmental stages of the plant. qRT‐PCR expression analyses and studies of EP3‐GFP and OsFBK1‐RFP promoter reporter lines demonstrate that although EP3 and OsFBK1 expression can be detected in the same tissues some cells exclusively express EP3 or OsFBK1 whilst others co‐express both genes. Loss, reduction or gain‐of‐function lines for EP3 and OsFBK1, show that EP3 and OsFBK1 affect plant architecture, organ size, floral organ number and size, floral morphology, pollen viability, grain size and weight. We have identified the putative orthologue genes of the rice microRNA pathway for ORYZA SATIVA DAWDLE (OsDDL) and ORYZA SATIVA SERRATE (OsSE), and demonstrated that EP3 and OsFBK1 affect their transcript levels as well as those of CROWN ROOT DEFECT 1/ORYZA SATIVA Exportin‐5 HASTY (CRD1/OsHST), ORYZA SATIVA DICER‐LIKE 1 (OsDCL) and ORYZA SATIVA WEAVY LEAF1 (OsWAF1). We show that EP3 affects OsPri‐MIR164, OsNAM1 and OsNAC1 transcript levels. OsNAC1 transcripts are modified by OsFBK1, suggesting two independent regulatory pathways, one via EP3 and OsMIR164 and the other via OsFBK1. Our data propose that EP3 and OsFBK1 conjointly play similar roles in rice to how HWS does in Arabidopsis. |
Databáze: | OpenAIRE |
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