Relaxin-Family Peptide Receptors 1 and 2 Are Fully Functional in the Bovine
| Autor: | Dana Janowski, Xuan Liu, Ravinder Anand-Ivell, Richard Ivell, Yanzhenzhi Dai |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty endocrine system Physiology Biology follicle lcsh:Physiology 03 medical and health sciences 0302 clinical medicine Physiology (medical) Internal medicine medicine INSL3 endometrium Receptor relaxin Original Research Relaxin 030219 obstetrics & reproductive medicine RXFP1 RXFP2 lcsh:QP1-981 urogenital system bovine HEK 293 cells Myometrium Transfection female genital diseases and pregnancy complications 030104 developmental biology Endocrinology Theca Relaxin/insulin-like family peptide receptor 2 hormones hormone substitutes and hormone antagonists Relaxin receptor |
| Zdroj: | Frontiers in Physiology, Vol 8 (2017) Frontiers in Physiology |
| ISSN: | 1664-042X |
| Popis: | In most mammals the peptide hormone relaxin is a key physiological component regulating early pregnancy and birth. However, synteny analysis shows that the gene encoding ovarian relaxin-2 is deleted in cows and sheep. While, these ruminants appear to exhibit a relaxin-like physiology, as in other mammals, until now a molecular understanding of this has been lacking. Cloning and expression analysis of the cognate bovine receptor for relaxin, RXFP1, as well as of the structurally related receptor, RXFP2, in female tissues, shows that these are expressed in a similar way to other mammals. RXFP1 transcripts are found in ovarian theca cells, endometrium, and myometrium, whereas RXFP2 transcripts are expressed in ovarian theca cells, oocytes, as well as in myometrium. Transfection of receptor-expressing gene constructs into HEK293 cells indicates that bovine RXFP1 has a greater EC50 at 10–50 nM for porcine or human relaxin, compared to human RXFP1. For bovine RXFP2, in contrast, the EC50 is |
| Databáze: | OpenAIRE |
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