A Pseudoautosomal Random Amplified Polymorphic DNA Marker for the Sex Chromosomes of Silene dioica
| Autor: | David L. Mulcahy, Verónica S. Di Stilio, Rick Kesseli |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Genetic Markers
DNA Plant Genotype Genetic Linkage Pseudoautosomal region Biology Genes Plant Y chromosome Magnoliopsida chemistry.chemical_compound Gene Frequency Molecular marker Genetics Small supernumerary marker chromosome Crosses Genetic DNA Primers Sex Chromosomes Base Sequence Bulked segregant analysis Random Amplified Polymorphic DNA Technique RAPD chemistry Nondisjunction Genetic marker Research Article |
| Zdroj: | Genetics. 149:2057-2062 |
| ISSN: | 1943-2631 |
| Popis: | The segregation pattern of an 810-bp random amplified polymorphic DNA (RAPD) band in the F1 and backcross generations of a Silene dioica (L.) Clairv. family provides evidence that this molecular marker is located in the pseudoautosomal region (PAR) of the X and Y chromosomes. The marker was found through a combination of bulked segregant analysis (BSA) and RAPD techniques. Recombination rates between this pseudoautosomal marker and the differentiating portion of the Y chromosome are 15% in both generations. Alternative explanations involving nondisjunction or autosomal inheritance are presented and discussed. Chromosome counts provide evidence against the nondisjunction hypothesis, and probability calculations argue against the possibility of autosomal inheritance. This constitutes the first report of a pseudoautosomal DNA marker for plant sex chromosomes. |
| Databáze: | OpenAIRE |
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