Plasma and Urinary Amino Acid-Derived Catabolites as Potential Biomarkers of Protein and Amino Acid Deficiency in Rats
| Autor: | Delphine Jouan-Rimbaud Bouveresse, Nadezda Khodorova, Joanna Moro, Jean-Charles Martin, Catherine Tardivel, Dalila Azzout-Marniche, Claire Gaudichon, Thierry Berton, Daniel Tomé |
|---|---|
| Přispěvatelé: | Physiologie de la Nutrition et du Comportement Alimentaire (PNCA (UMR 0914)), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), platform BIOMET, Supported by the UMR Nutrition Physiology and Ingestive Behavior. AlimH department of Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement., Azzout-Marniche, Dalila |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
D. Plasma and Urinary Amino Acid-Derived Catabolites as protein
0301 basic medicine Nitrogen balance [SDV.MHEP.PHY] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] Metabolite [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Urine chemistry.chemical_compound Protein Deficiency [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] TX341-641 Food science Azzout-Marniche Essential amino acid [SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism 2. Zero hunger chemistry.chemical_classification Nutrition and Dietetics T Discriminant Analysis [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular Networks [q-bio.MN] deficiency [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism Martin metabolomics Amino acid D Dietary Proteins J.-C amino acid requirement Weanling Article Gaudichon C 03 medical and health sciences Metabolomics Tardivel [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] Khodorova Animals Least-Squares Analysis Analysis of Variance 030109 nutrition & dietetics Catabolism Nutrition. Foods and food supply Nutritional Requirements J biomarkers [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology N Animal Feed Rats Disease Models Animal [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition Nutrition Assessment 030104 developmental biology chemistry Jouan-Rimbaud Bouveresse Berton Multivariate Analysis Tomé Amino Acids Essential Deficiency Diseases protein [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Moro Food Science |
| Zdroj: | Nutrients, Vol 13, Iss 1567, p 1567 (2021) Nutrients Nutrients, MDPI, 2021, 13 (1567), pp.1-14. ⟨10.3390/nu13051567⟩ Nutrients, 2021, 13 (5), pp.1567. ⟨10.3390/nu13051567⟩ Volume 13 Issue 5 |
| ISSN: | 2072-6643 |
| DOI: | 10.3390/nu13051567⟩ |
| Popis: | International audience; Objective: Dietary intakes must cover protein and essential amino acid (EAA) requirements. For this purpose, different methods have been developed such as the nitrogen balance method, factorial method, or AA tracer studies. However, these methods are either invasive or imprecise, and the Food and Agriculture Organization of the United Nations (FAO, 2013) recommends new methods and, in particular, metabolomics. The aim of this study is to determine total protein/EAA requirement in the plasma and urine of growing rats. Methods: 36 weanling rats were fed with diets containing 3, 5, 8, 12, 15, and 20% protein for 3 weeks. During experimentation, urine was collected using metabolic cages, and blood from the portal vein and vena was taken at the end of the experiment. Metabolomics analyses were performed using LC-MS, and the data were analyzed with a multivariate analysis model, partial least Squares (PLS) regression, and independent component-discriminant analysis (ICDA). Each discriminant metabolite identified by PLS or ICDA was tested by one-way ANOVA to evaluate the effect of diet. Results: PLS and ICDA allowed us to identify discriminating metabolites between different diet groups. Protein deficiency led to an increase in the AA catabolism enzyme systems inducing the production of breakdown metabolites in the plasma and urine. Conclusion: These results indicate that metabolites are specific for the state of EAA deficiency and sufficiency. Some types of biomarkers such as AA degradation metabolites appear to be specific candidates for protein/EAA requirement. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|