Research on anti-hepatocellular carcinoma activity and mechanism of Polygala fallax Hemsl
| Autor: | Hui Tang, Tiexin Zeng, Hongfei Gong, Zhiwei Wang, Kaimei Zhu, Shengjiu Gu, Yuanyuan Lan, Zhiren Yao, Yu Li |
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| Rok vydání: | 2019 |
| Předmět: |
MAPK/ERK pathway
Carcinoma Hepatocellular Polygala Apoptosis Cell Cycle Proteins Acetates Flow cytometry 03 medical and health sciences Inhibitory Concentration 50 0302 clinical medicine Western blot Drug Discovery medicine Humans IC50 Protein kinase B 030304 developmental biology Cell Proliferation Pharmacology 0303 health sciences medicine.diagnostic_test Dose-Response Relationship Drug Chemistry Plant Extracts Liver Neoplasms Hep G2 Cells Molecular biology Antineoplastic Agents Phytogenic Gene Expression Regulation Neoplastic Real-time polymerase chain reaction medicine.anatomical_structure 030220 oncology & carcinogenesis Hepatocyte Solvents Apoptosis Regulatory Proteins Signal Transduction |
| Zdroj: | Journal of ethnopharmacology. 260 |
| ISSN: | 1872-7573 |
| Popis: | Ethnopharmacological relevance Polygala fallax Hemsl. is a kind of traditional medicine of Yao Minority in China. In Chinese medicine practice, Polygala fallax Hemsl. is commonly prescribed to treat all kinds of acute and chronic hepatitis. Aim of the study The present study aimed at investigating the effects and its possible mechanism of Polygala fallax Hemsl. on the proliferation and apoptosis of HepG2 cells (a kind of human hepatoma cell). Materials and methods Through a variety of experimental methods, including MTT technique and Hoechst staining to detect apoptosis in Hepatocyte HepG2 cells, flow cytometry to observe the pro-apoptotic and circulatory arrest effects as well as real-time fluorescence quantitative polymerase chain reaction (q-PCR) technique to examine the expression levels of Bcl-2/Bax gene and prote Western blot to examine the expression levels of bcl-2/bax,caspase3,8,9,CyclinA,p21,p27,ERK.Phospho-ERK and AKT, Phospho-AKT in HepG2 cells. Results The results showed that compared with the control group, all polarity fractions of P. fallax had inhibitory effects on HepG2 cells, among which the inhibition effect of ethyl acetate fraction in 0.036 ± 0.001 mg/mL of IC50 for 24 h was the most obvious (P Conclusion It could be concluded that the ethyl acetate fraction of Polygala fallax Hemsl. can significantly prohibit the proliferation of HepG2 cells. The possible mechanism is to promote the expression of Bax, inhibit the expression of Bcl-2, and down regulate the expression of AKT and ERK. |
| Databáze: | OpenAIRE |
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