Stage-dependent localization of LHCP II apoprotein in the Golgi of synchronized cells of Euglena gracilis by immunogold electron microscopy
| Autor: | Tetsuaki Osafune, Eiji Hase, Jerome A. Schiff |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Chlorophyll
Chloroplasts Euglena gracilis Macromolecular Substances Photosynthetic Reaction Center Complex Proteins ved/biology.organism_classification_rank.species Mutant Light-Harvesting Protein Complexes Protozoan Proteins Golgi Apparatus Biology law.invention symbols.namesake law polycyclic compounds Animals Photosynthesis Plant Proteins Antiserum ved/biology Cell Cycle Photosystem II Protein Complex food and beverages Cell Biology Immunogold labelling Cell cycle Golgi apparatus Immunohistochemistry Cell Compartmentation Microscopy Electron Biochemistry Thylakoid symbols Biophysics lipids (amino acids peptides and proteins) Electron microscope Apoproteins Protein Processing Post-Translational Euglena |
| Zdroj: | Experimental Cell Research. 193:320-330 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(91)90103-2 |
| Popis: | We have localized LHCP II apoprotein in the Golgi and thylakoids of Euglena gracilis Klebs var. bacillaris Cori and strain Z Pringsheim by electron microscopy using a specific antibody and protein A-gold. Using synchronized cells (light, 14 h:dark, 10 h) we show that thylakoids are always immunoreactive. There is no reaction in the Golgi at 0 h (the beginning of the light period) but immunoreaction appears in the Golgi soon thereafter, rises to a peak at 8 h and declines to zero by 16 h (2 h into the dark period). The peak in immunoreaction in the Golgi immediately precedes the peak in cellular 14C-labeling of thylakoid LHCP II apoprotein seen by Brandt and von Kessel (Plant Physiol. (1983) 72, 616), supporting our suggestion that processing in the Golgi precedes deposition of LHCP apoprotein in the thylakoids. Substitution of preimmune serum for antiserum eliminates the immunoreaction in the Golgi, and thylakoids of synchronized cells of mutant Gr1BSL which lacks LHCP II apoprotein show no immunoreaction in the Golgi or thylakoids at any stage. Random observations indicate that the compartmentalized osmiophilic structure (COS) shows an immunoreaction with anti-LHCP II apoprotein antibody at 1 h into the light period (when the Golgi is not immunoreactive) and at 10 h into the light period (when the Golgi is fully reactive), suggesting that the COS remains immunoreactive throughout the cell cycle. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|