5-HT1A receptors increase excitability of spinal motoneurons by inhibiting a TASK-1-like K+ current in the adult turtle
| Autor: | Perrier, Jean-François, Alaburda, Aidas, Hounsgaard, Jørn |
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| Rok vydání: | 2003 |
| Předmět: |
Motor Neurons
8-Hydroxy-2-(di-n-propylamino)tetralin Serotonin Patch-Clamp Techniques Potassium Channels Polyunsaturated Alkamides Physiology musculoskeletal neural and ocular physiology Nerve Tissue Proteins Original Articles Arachidonic Acids In Vitro Techniques Membrane Potentials Serotonin Receptor Agonists Turtles Electrophysiology Potassium Channels Tandem Pore Domain nervous system Spinal Cord Receptor Serotonin 5-HT1A polycyclic compounds Potassium Channel Blockers Animals heterocyclic compounds Endocannabinoids |
| Zdroj: | The Journal of Physiology. 548:485-492 |
| ISSN: | 0022-3751 |
| DOI: | 10.1113/jphysiol.2002.037952 |
| Popis: | The modulatory effects of serotonin mediated by 5-HT1A receptors in adult spinal motoneurons were investigated by intracellular recordings in a slice preparation from the turtle. In current-clamp mode, activation of 5-HT1A receptors by 8-OH-DPAT led to depolarization and an increase in input resistance in most motoneurons but caused hyperpolarization and a decrease in input resistance in the remaining smaller fraction of cells. When slices were preincubated in medium containing the 5-HT1A receptor antagonist WAY-100635, 8-OH-DPAT had no effect. In voltage-clamp mode, with 1 mM CsCl in the bathing medium, 8-OH-DPAT consistently inhibited a leak current that was sensitive to extracellular acidification and anandamide, a TASK-1 channel blocker. In medium with a low pH, as in the presence of anandamide, 8-OH-DPAT had no effect. Our results show that activation of 5-HT1A receptors contributes to the excitatory effect of serotonin on spinal motoneurons by inhibition of a TASK-1 potassium channel leading to depolarization and increased input resistance. |
| Databáze: | OpenAIRE |
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