Exploring integument transcriptomes, cuticle ultrastructure, and cuticular hydrocarbons profiles in eusocial and solitary bee species displaying heterochronic adult cuticle maturation

Autor:	Moysés Elias-Neto, Fabiano Carlos Pinto de Abreu, Carlos A. Garófalo, Márcia Maria Gentile Bitondi, Izabel Cristina Casanova Turatti, Marcela A.F.B. Laure, Juliana S. Galaschi-Teixeira, Maria Juliana Ferreira-Caliman, Michelle P.M. Soares, Vera Lucia Castelo Figueiredo, Zilá Luz Paulino Simões, Tiago Falcon, Daniel Guariz Pinheiro, Norberto Peporine Lopes, Juliana Martins
Přispěvatelé:	Universidade de São Paulo (USP), Hosp Clin Porto Alegre, Universidade Estadual Paulista (Unesp)
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	0106 biological sciences 0301 basic medicine Odorant binding Physiology Polymers QUITINA Gene Identification and Analysis Gene Expression Chitin Genetic Networks Molting 01 natural sciences Medicine and Health Sciences Evolutionary Emergence Materials media_common Multidisciplinary Metamorphosis Biological Eukaryota Bees Insects Chemistry Epidermis (zoology) Macromolecules Physical Sciences Insect Proteins Medicine Female Integument Integumentary System Honey Bees Heterochrony Moulting Network Analysis Research Article Computer and Information Sciences Evolutionary Processes Arthropoda media_common.quotation_subject Cuticle Science Materials Science Zoology Biology 03 medical and health sciences Genetics Animals Gene Regulation Metamorphosis Evolutionary Biology Organisms Biology and Life Sciences Polymer Chemistry Invertebrates Hymenoptera Hydrocarbons 010602 entomology 030104 developmental biology Ultrastructure Epidermis Transcriptome Physiological Processes
Zdroj:	PLoS ONE, Vol 14, Iss 3, p e0213796 (2019) Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP PLoS ONE
ISSN:	1932-6203
Popis:	Made available in DSpace on 2019-10-04T12:36:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-03-14 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Differences in the timing of exoskeleton melanization and sclerotization are evident when comparing eusocial and solitary bees. This cuticular maturation heterochrony may be associated with life style, considering that eusocial bees remain protected inside the nest for many days after emergence, while the solitary bees immediately start outside activities. To address this issue, we characterized gene expression using large-scale RNA sequencing (RNA-seq), and quantified cuticular hydrocarbon (CHC) through gas chromatography-mass spectrometry in comparative studies of the integument (cuticle plus its underlying epidermis) of two eusocial and a solitary bee species. In addition, we used transmission electron microscopy (TEM) for studying the developing cuticle of these and other three bee species also differing in life style. We found 13,200, 55,209 and 30,161 transcript types in the integument of the eusocial Apis mellifera and Frieseomelitta varia, and the solitary Centris analis, respectively. In general, structural cuticle proteins and chitin-related genes were upregulated in pharate-adults and newly-emerged bees whereas transcripts for odorant binding proteins, cytochrome P450 and antioxidant proteins were overrepresented in foragers. Consistent with our hypothesis, a distance correlation analysis based on the differentially expressed genes suggested delayed cuticle maturation in A. mellifera in comparison to the solitary bee. However, this was not confirmed in the comparison with F. varia. The expression profiles of 27 of 119 genes displaying functional attributes related to cuticle formation/differentiation were positively correlated between A. mellifera and F. varia, and negatively or non-correlated with C. analis, suggesting roles in cuticular maturation heterochrony. However, we also found transcript profiles positively correlated between each one of the eusocial species and C. analis. Gene co-expression networks greatly differed between the bee species, but we identified common gene interactions exclusively between the eusocial species. Except for F. varia, the TEM analysis is consistent with cuticle development timing adapted to the social or solitary life style. In support to our hypothesis, the absolute quantities of n-alkanes and unsaturated CHCs were significantly higher in foragers than in the earlier developmental phases of the eusocial bees, but did not discriminate newly-emerged from foragers in C. analis. By highlighting differences in integument gene expression, cuticle ultrastructure, and CHC profiles between eusocial and solitary bees, our data provided insights into the process of heterochronic cuticle maturation associated to the way of life. Univ Sao Paulo, Dept Genet, Fac Med Ribeirao Preto, Ribeirao Preto, Brazil Hosp Clin Porto Alegre, Nucleo Bioinformat, Porto Alegre, RS, Brazil Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Tecnol, Jaboticabal, Brazil Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ribeirao Preto, Brazil Univ Sao Paulo, Dept Fis & Quim, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto, Brazil Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Tecnol, Jaboticabal, Brazil FAPESP: 2014/13136-0 FAPESP: 2011/03171-5 FAPESP: 2014/50265-3 CAPES: 88887.160608/2017-00 CAPES: 001 : FAPESP 2012/24284-5
Databáze:	OpenAIRE
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