18β-Glycyrrhetinic acid induces immunological adjuvant activity of Th1 against Candida albicans surface mannan extract
| Autor: | Jeonghyeon Kim, Inkyung Joo, Hayan Kim, Yongmoon Han |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_treatment
T cell Freund's Adjuvant Pharmaceutical Science chemical and pharmacologic phenomena Spleen Mannans Interferon-gamma Mice Th2 Cells Adjuvants Immunologic Candida albicans Drug Discovery Glycyrrhiza medicine Animals Edema Hypersensitivity Delayed Th1-Th2 Balance Cell Proliferation Mannan Pharmacology Mice Inbred BALB C biology Plant Extracts Candidiasis Th1 Cells biology.organism_classification Lipids Molecular biology Corpus albicans Cytokine medicine.anatomical_structure Complementary and alternative medicine Polyclonal antibodies Immunoglobulin G Immunology biology.protein Glycyrrhetinic Acid Molecular Medicine Female Immunization Interleukin-4 Adjuvant Phytotherapy |
| Zdroj: | Phytomedicine. 20:951-955 |
| ISSN: | 0944-7113 |
| DOI: | 10.1016/j.phymed.2013.04.008 |
| Popis: | The aim of this study was to determine the immunological adjuvant effect of 18β-glycyrrhetinic acid (GA) isolated from Glycyrrhizae radix. In the experiments, BALB/c mice were immunized on days 1 and 22 intraperitoneally (i.p.) with an emulsion form of Candida albicans surface mannan extract (SM) mixed with either Incomplete Freund's Adjuvant [SM/IFA], or Complete Freund's Adjuvant [SM/CFA] or GA mixed with IFA [SM/GA/IFA]. One week after the second immunization, polyclonal sera were collected from these animals in order to determine IgG isotypes and cytokine profiles in the sera. After the collection, the spleen samples were collected to determine the degree of T cell proliferation. Additionally, the DTH (delayed type hypersensitivity) response was examined by measuring the footpad swelling of immunized mice. Data resulting from the T cell proliferation test showed that SM/GA/IFA enhanced the proliferation the most. The enhancement was about 85% more compared to SM/IFA (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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