Alteration of Adaptive Immunity in a Colorectal Peritoneal Carcinomatosis Model
| Autor: | Hongming Miao, Xia Kang, Wei Xiang, Rongchen Shi, Lili Zhang, Jinping Wang, Fengtian He |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Colorectal cancer business.industry peritoneal carcinomatosis Intraperitoneal inoculation colorectal cancer Spleen adaptive immunity medicine.disease Acquired immune system Colorectal cancer cell line Peritoneal carcinomatosis 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis medicine Cancer research business Visceral fat CD8 Research Paper |
| Zdroj: | Journal of Cancer |
| ISSN: | 1837-9664 |
| DOI: | 10.7150/jca.27947 |
| Popis: | Colorectal cancer (CRC) usually gives rise to transcoelomic spread and ultimately causes peritoneal carcinomatosis (PC). However, mechanism studies, especially the immunological basis of colorectal PC, are rarely revealed due to lack of a suitable PC model. Here we selected a mouse colorectal cancer cell line MC-38 for intraperitoneal inoculation in the C57BL/6 mice to mimic the development of colorectal PC. We demonstrated that the injected CRC cells preferentially and rapidly migrated and colonized in the visceral fat tissues, but not in other visceral organs. With flow cytometric analysis, we found the proportions of spleen T cells and B cells were not affected by PC progression, while the ratios of blood CD4+ and CD8+ T cells were largely influenced. Especially, the quantity or activity of CD4+ and CD8+ T cells in visceral fats were intimately regulated by PC development. Taken together, we successfully constructed a colorectal PC model in immune-competent mice and revealed the alteration of adaptive immunity in PC development. Our study might potentiate the research and therapy strategies of colorectal PC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|