Peroxisomes Extend Peroxules in a Fast Response to Stress via a Reactive Oxygen Species-Mediated Induction of the Peroxin PEX11a
| Autor: | Luisa M. Sandalio, María C. Romero-Puertas, Jianping Hu, María Sanz-Fernández, María Rodríguez-Serrano |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Research Articles - Focus Issue Arabidopsis Peroxisome Proliferation Peroxin Plant Science 01 natural sciences 03 medical and health sciences Stress Physiological RNA interference Peroxisomes Genetics chemistry.chemical_classification Reactive oxygen species NADPH oxidase biology Arabidopsis Proteins fungi Membrane Proteins NADPH Oxidases Peroxisome biology.organism_classification Cell biology Plant Leaves 030104 developmental biology chemistry biology.protein Lipid Peroxidation Signal transduction Reactive Oxygen Species Cadmium Signal Transduction 010606 plant biology & botany |
| Zdroj: | Plant Physiology. 171:1665-1674 |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.16.00648 |
| Popis: | Peroxisomes are highly dynamic and metabolically active organelles that play an important role in cellular functions, including reactive oxygen species (ROS) metabolism. Peroxisomal dynamics, such as the proliferation, movement, and production of dynamic extensions called peroxules, have been associated with ROS in plant cells. However, the function and regulation of peroxules are largely unknown. Using confocal microscopy, we have shown that treatment of Arabidopsis leaves with the heavy metal cadmium produces time course-dependent changes in peroxisomal dynamics, starting with peroxule formation, followed by peroxisome proliferation, and finally returning to the normal morphology and number. These changes during Cd treatment were regulated by NADPH oxidase (C and F)-related ROS production. Peroxule formation is a general response to stimuli such as arsenic and is regulated by peroxin 11a (PEX11a), as Arabidopsis pex11a RNAi lines are unable to produce peroxules under stress conditions. The pex11a line showed higher levels of lipid peroxidation content and lower expression of genes involved in antioxidative defenses and signaling, suggesting that these extensions are involved in regulating ROS accumulation and ROS-dependent gene expression in response to stress. Our results demonstrate that PEX11a and peroxule formation play a key role in regulating stress perception and fast cell responses to environmental cues. |
| Databáze: | OpenAIRE |
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