Isolation of acini from nasal glands of the guinea-pig
| Autor: | Yoshitaka Saito, Yukio Katori, Akinori Nishiyama, Hiroshi Sunose, Katsuhisa Ikeda, Masaaki Suzuki, W. Zhang, Motoaki Ishigaki, Tomonori Takasaka |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Atropine
Pathology medicine.medical_specialty Patch-Clamp Techniques Physiology Guinea Pigs In Vitro Techniques Biology Membrane Potentials Guinea pig chemistry.chemical_compound stomatognathic system Acinus Parasympathetic Nervous System medicine Animals Patch clamp Serous gland Nasal Septum Staining and Labeling Histocytochemistry Nasal glands Molecular biology Acetylcholine Microscopy Electron Nasal Mucosa medicine.anatomical_structure Microscopy Fluorescence chemistry Collagenase Calcium Trypan blue Cytophotometry Fura-2 Signal Transduction medicine.drug |
| Zdroj: | Acta Physiologica Scandinavica. 151:377-384 |
| ISSN: | 1365-201X 0001-6772 |
| DOI: | 10.1111/j.1748-1716.1994.tb09757.x |
| Popis: | A procedure for isolating the acinar cells of the serous gland in the mammalian nasal septum has been developed. This technique is characterized by meticulous and selective isolation with minimal contamination by the surface epithelial cells and employs enzymatic treatment with collagenase. The isolated cells were confirmed to be serous gland acini as shown by negative staining with Alcian blue and a high electron density of the granules. The acini were more than 90% viable as judged by trypan blue exclusion. Ultrastructural integrity of the cells was well maintained following the isolation procedure. Application of acetylcholine to the isolated acini induced an inward current in a whole-cell patch clamp and increased intracellular Ca2+ concentration measured by fura-2. These acetylcholine responses were completely blocked by atropine. These physiological findings directly demonstrated that nasal gland acini possess muscarinic-activated receptors as previously suggested. These isolated cells hold promise for the in vitro study of secretory mechanisms in the mammalian nasal gland. |
| Databáze: | OpenAIRE |
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