Recovery byN-acetylcysteine from subchronic exposure to Imidacloprid-induced hypothalamic–pituitary–adrenal (HPA) axis tissues injury in male rats

Autor:	Narges El Golli, Najoua Gharbi, Ines Bini Dhouib, Saloua El Fazâa, Aicha Jrad Lamine, Alya Annabi, Mohamed Montassar Lasram
Rok vydání:	2015
Předmět:	Male Hypothalamo-Hypophyseal System Insecticides medicine.medical_specialty Health Toxicology and Mutagenesis Pituitary-Adrenal System Toxicology medicine.disease_cause Synaptic Transmission Antioxidants Acetylcysteine Superoxide dismutase Neonicotinoids chemistry.chemical_compound Parasympathetic Nervous System Imidacloprid Internal medicine Adrenal Glands parasitic diseases medicine Animals Rats Wistar biology Chemistry Imidazoles Neonicotinoid Organ Size Nitro Compounds Malondialdehyde Rats Oxidative Stress Nicotinic acetylcholine receptor Cholesterol Endocrinology biology.protein Cholinergic Calcium Oxidative stress medicine.drug
Zdroj:	Toxicology Mechanisms and Methods. 25:524-531
ISSN:	1537-6524 1537-6516
DOI:	10.3109/15376516.2015.1045663
Popis:	Imidacloprid is the most important example of the neonicotinoid insecticides known to target the nicotinic acetylcholine receptor in insects, and potentially in mammals. N-Acetyl-l-cysteine (NAC) has been shown to possess curative effects in experimental and clinical investigations. The present study was designed to evaluate the recovery effect of NAC against Imidacloprid-induced oxidative stress and cholinergic transmission alteration in hypothalamic-pituitary-adrenal (HPA) axis of male rats following subchronic exposure. About 40 mg/kg of Imidacloprid was administered daily by intragastric intubation and 28 days later, the rats were sacrificed and HPA axis tissues were removed for different analyses. Imidacloprid increased adrenal relative weight and cholesterol level indicating an adaptive stage of the general alarm reaction to stress. Moreover, Imidacloprid caused a significant increase in malondialdehyde level, the antioxidants catalase, superoxide dismutase and glutathione-S-transferase showed various alterations following administration and significant depleted thiols content was only recorded in hypothalamic tissue. Furthermore, the hypothalamic and pituitary acetylcholinesterase activity and calcium level were significantly increased highlighting the alteration of cholinergic activity. The present findings revealed that HPA axis is a sensitive target to Imidacloprid (IMI). Interestingly, the use of NAC for only 7 days post-exposure to IMI showed a partial therapeutic effect against Imidacloprid toxicity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0fd76f775aee31b2c5518486d4b1995 https://doi.org/10.3109/15376516.2015.1045663 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.