Dietary fish oil supplements modify ruminal biohydrogenation, alter the flow of fatty acids at the omasum, and induce changes in the ruminal Butyrivibrio population in lactating cows
| Autor: | Seppo Ahvenjärvi, Stefan M. Muetzel, Aila Vanhatalo, Kevin J. Shingfield, Vesa Toivonen, R. John Wallace, Delphine Paillard, Piia Kairenius, Pekka Huhtanen, Anu Ärölä, J. Mikko Griinari |
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| Rok vydání: | 2012 |
| Předmět: |
Omasum
Rumen Conjugated linoleic acid Population Medicine (miscellaneous) Biology Polymerase Chain Reaction Sensitivity and Specificity 03 medical and health sciences chemistry.chemical_compound Animal science Fish Oils Butyrivibrio Animals Lactation education 2. Zero hunger chemistry.chemical_classification 0303 health sciences education.field_of_study Nutrition and Dietetics Cross-Over Studies Dose-Response Relationship Drug 030306 microbiology Fatty Acids 0402 animal and dairy science Fatty acid 04 agricultural and veterinary sciences Fish oil biology.organism_classification 040201 dairy & animal science Animal Feed Diet chemistry Biochemistry Dietary Supplements Animal Nutritional Physiological Phenomena Cattle Female Polyunsaturated fatty acid |
| Zdroj: | The Journal of nutrition. 142(8) |
| ISSN: | 1541-6100 |
| Popis: | Four lactating cows fitted with ruminal cannulae and fed a grass silage-based diet were used in a 4 3 4 Latin square with 28-d periods to investigate the effects of incremental dietary fish oil (FO) supplementation (0, 75, 150, or 300 g/d) on the flow of fatty acids at the omasum and populations of rumen bacteria capable of biohydrogenation. FO decreased silage intake and ruminal volatile fatty acid concentrations and promoted an increase in molar butyrate and propionate proportions at the expense of acetate. Extensive ruminal biohydrogenation of 20:5(n-3) and 22:6(n-3) resulted in corresponding increases in numerous 20- and 22-carbon unsaturated fatty acids at the omasum. Omasal flow of several 20-, 21-, and 22-carbon all-cis (n-3) PUFA exceeded the intake from FO. Supplements of FO also induced a dose-dependent decrease in 18:0 and increased trans 18:1 and trans 18:2 flow at the omasum. Trans-11 was the major 18:1 intermediate in digesta, while FO induced quadratic increases intrans-10 18:1flow, reachinga maximum of 300 g/d. FO had no substantial influence on omasal flow of CLA. Results suggest that one or more fatty acids in FO inhibit the reduction of trans-18:1 and trans-18:2 intermediates by ruminal microorganisms. qPCR based on 16S rRNA genes in omasal digesta indicated that key Butyrivibrio spp. declined linearly in response to FO. Dose-dependent increases in ruminal outflow of biohydrogenation intermediates containing one or more trans double bonds in response to FO has major implications for host metabolism and the nutritional quality of ruminant foods. J. Nutr. 142: 1437‐1448, 2012. |
| Databáze: | OpenAIRE |
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