CETP Gene may be Associated with Advanced Age-Related Macular Degeneration in the Chinese Population
| Autor: | Jie Zhou, Xiaoming Hou, Kang Zhang, Guoxian Qiu, Guiqun Cao, Ming Zhang, Zhiguang Su, Dan Wang, Duy H. Nguyen, Gen Li |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Oncology China medicine.medical_specialty Genotyping Techniques Single-nucleotide polymorphism Biology Bioinformatics Polymerase Chain Reaction Polymorphism Single Nucleotide law.invention Macular Degeneration Asian People Gene Frequency law Internal medicine Genotype medicine Humans Allele Genetics (clinical) Polymerase chain reaction Aged Lipase Middle Aged Macular degeneration medicine.disease eye diseases Cholesterol Ester Transfer Proteins Lipoprotein Lipase Ophthalmology ABCA1 Pediatrics Perinatology and Child Health HTRA1 biology.protein Female lipids (amino acids peptides and proteins) ATP Binding Cassette Transporter 1 Lipoprotein |
| Zdroj: | Ophthalmic Genetics. 36:303-308 |
| ISSN: | 1744-5094 1381-6810 |
| DOI: | 10.3109/13816810.2014.881506 |
| Popis: | Objectives: This study aims to investigate whether variations in LIPC, CETP, ABCA1 and LPL, which are involved in high-density lipoprotein (HDL) metabolism, are associated with advanced age-related macular degeneration (AMD) in the Chinese population.Design and Methods: A total of 119 Chinese patients with advanced AMD and 99 control individuals were recruited. Genomic DNA was extracted from peripheral blood leukocytes. Genotypes of seven single nucleotide polymorphisms (SNPs) including rs1061170 and rs1410996 in CFH, rs10490924 in HTRA1, rs10468017 in LIPC, rs3764261 in CETP, rs1883025 in ABCA1 and rs12678919 near LPL were determined by polymerase chain reaction (PCR) followed by allele-specific restriction enzyme digestion or SNaPshot. Unconditional logistic regression analyses were performed to generate a risk predictive model.Results: We observed the frequency of allele A of rs3764261 in CETP to be significantly lower in advanced AMD after Bonferroni correction (15.5% in patients with AMD and 20.7% in controls; OR = 0.49, 95% CI: 0.29–0.85; p = 0.011). Furthermore, we found that it was also associated with reduced risk of both unilateral AMD (OR = 0.52, 95% CI: 0.28–0.98; p = 0.043) and bilateral AMD (OR = 0.45, 95% CI: 0.22–0.91; p = 0.026). Rs10468017 in LIPC, rs12678919 near LPL and rs1883025 in ABCA1 were not found to be associated with advanced AMD (all p > 0.05).Conclusion: Our data suggested that the allele A in rs3764261 in CETP gene may be associated with a decreased risk of advanced AMD in Chinese population. |
| Databáze: | OpenAIRE |
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