Solution-state behaviour of algal mono-uronates evaluated by pure shift and compressive sampling NMR techniques
| Autor: | Philip W. Dyer, Jack S. Rowbotham, H. Christopher Greenwell, Juan A. Aguilar, Alan M. Kenwright |
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| Rok vydání: | 2020 |
| Předmět: |
Magnetic Resonance Spectroscopy
010405 organic chemistry Solution state Chemistry Organic Chemistry Molecular Conformation General Medicine 010402 general chemistry 01 natural sciences Biochemistry 0104 chemical sciences Analytical Chemistry Sodium salt Solutions Uronic Acids Pyranose Computational chemistry High resolution spectra Biomass Spectroscopy |
| Zdroj: | Carbohydrate research, 2020, Vol.495, pp.108087 [Peer Reviewed Journal] |
| Popis: | Sodium salts of the algal uronic-acids, d-mannuronic acid (HManA) and l-guluronic acid (HGulA) have been isolated and characterised in solution by nuclear magnetic resonance (NMR) spectroscopy. A suite of recently-described NMR experiments (including pure shift and compressive sampling techniques) were used to provide confident assignments of the pyranose forms of the two uronic acids at various pD values (from 7.5 to 1.4). The resulting high resolution spectra were used to determine several previously unknown parameters for the two acids, including their pKa values, the position of their isomeric equilibria, and their propensity to form furanurono-6,3-lactones. For each of the three parameters, comparisons are drawn with the behaviour of the related D-glucuronic (HGlcA) and D-galacturonic acids (HGalA), which have been previously studied extensively. This paper demonstrates how these new NMR spectroscopic techniques can be applied to better understand the properties of polyuronides and uronide-rich macroalgal biomass. |
| Databáze: | OpenAIRE |
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