Cloning and characterization of SOB1, a new testis-specific cDNA encoding a human sperm protein probably involved in oocyte recognition
| Autor: | C. Finaz, Clotilde Duquenne, E. Rogier, M.F. Rousseau-Merck, Annick Lefèvre |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
DNA Complementary Sequence analysis Molecular Sequence Data Biophysics A Kinase Anchor Proteins Biology Biochemistry law.invention Evolution Molecular law Complementary DNA Cricetinae Testis Animals Humans Northern blot Amino Acid Sequence RNA Messenger Cloning Molecular Molecular Biology Polymerase chain reaction Adaptor Proteins Signal Transducing Cloning Sperm-Ovum Interactions Two-dimensional gel electrophoresis Chromosomes Human Pair 12 Base Sequence Reverse Transcriptase Polymerase Chain Reaction Binding protein Chromosome Mapping Cell Biology Molecular biology Spermatozoa Peptide Fragments Open reading frame Gene Expression Regulation Oocytes Female Carrier Proteins |
| Zdroj: | Biochemical and biophysical research communications. 259(1) |
| ISSN: | 0006-291X |
| Popis: | A human sperm-oocyte binding protein, SOB1, was purified by two dimensional gel electrophoresis and sequenced. This protein was selected because it was recognized by a monoclonal antibody that inhibited the binding of human sperm to zona-free hamster oocytes. The sequences of the tryptic peptides were used to design degenerate primers. These were used to amplify a specific fragment from human testis cDNA by the polymerase chain reaction. This 1233 bp fragment was extended in 3′ and 5′ by RACE to obtain the 3 kb full length SOB1 cDNA. Sequence analysis indicated that the deduced open reading frame encodes a 853 amino acid protein, with a molecular mass of 94.7 kDa. This is a new testis-specific cDNA. It is 27, 32.8 and 34.4% homologous to three sperm proteins, HI, Fsc1 and AKAP82 respectively. A single 3kb transcript was demonstrated only in the testis by northern blot analysis. It is a single copy gene, well conserved among mammals and located on human chromosome 12 at band p13. |
| Databáze: | OpenAIRE |
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