Cultured Human Bladder Tumors for Pharmacodynamic Studies
| Autor: | M. Guillaume Wientjes, Thomas D. Schmittgen, Jessie L.-S. Au, Joseph R. Drago, Robert A. Badalament |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Pathology
medicine.medical_specialty Mitomycin Urology Antineoplastic Agents Mitomycins Tumor Cells Cultured Carcinoma medicine Humans Viability assay Carcinoma Transitional Cell Urinary bladder Dose-Response Relationship Drug business.industry Mitomycin C medicine.disease Molecular biology In vitro Dose–response relationship Transitional cell carcinoma medicine.anatomical_structure Urinary Bladder Neoplasms Cell culture Carcinoma Squamous Cell Autoradiography Drug Screening Assays Antitumor business |
| Zdroj: | Journal of Urology. 145:203-207 |
| ISSN: | 1527-3792 0022-5347 |
| DOI: | 10.1016/s0022-5347(17)38292-7 |
| Popis: | Human bladder tumor fragments were cultured on collagen gel. In this system, the three dimensional architecture, cell-to-stroma and cell-to-cell interactions, and tumor heterogeneity were maintained. Cell viability and labeling index (LI) were determined by exposure to 3H-thymidine and autoradiography. Of the samples from 20 patients with transitional cell carcinoma, 14 (70%) were successfully cultured and had a mean LI of 32%. In addition, one specimen from a patient with squamous cell carcinoma was cultured and had a LI of 61%. Cultured samples were tested for chemosensitivity using a two hour exposure of mitomycin C in concentrations ranging from one to 50 micrograms./ml. A dose-dependent relationship was demonstrated; LI decreased as mitomycin C concentrations increased. The methodology described provides an alternative to suspension or monolayer techniques of culturing human bladder tumors for pharmacological studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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