Insulin Preincubation Effects on Rat Vessel Contractile Responses: Role of the Endothelium
| Autor: | A. O. Grassi de Gende, G. J. Rinaldi, A. Gómez Alvis, Verónica Milesi, Alejandro Rebolledo |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
Tail medicine.medical_specialty Vasopressin Contraction (grammar) Endothelium Physiology medicine.medical_treatment Receptors Thromboxane Vasodilation Biology Muscle Smooth Vascular Hyperinsulinism Internal medicine medicine Animals Insulin Drug Interactions Enzyme Inhibitors Rats Wistar Aorta Endothelin-1 Antagonist Cell Biology General Medicine Protein-Tyrosine Kinases Endothelin 1 Rats Arginine Vasopressin Insulin receptor Endocrinology medicine.anatomical_structure Prostaglandin-Endoperoxide Synthases Vasoconstriction biology.protein Endothelium Vascular Nitric Oxide Synthase |
| Zdroj: | Endothelium. 8:269-276 |
| ISSN: | 1029-2373 1062-3329 |
| DOI: | 10.3109/10623320109090804 |
| Popis: | The effect of contractions elicited with ET1 and AVP after preincubating rat aortic and tail artery rings with a hyperinsulinemic dose (3 nM) of insulin were studied. Insulin preincubation (120 min), in the presence of 0.1 mM L-NAME, depressed contraction of aortic rings to 0.01 microM ET1 (132 +/- 6 vs. 161 +/- 9 mg/mm2 in control, n = 25; p0.05) and to 1 microM AVP (84 +/- 7 vs. 110 +/- 9 mg/mm2 in control, n = 16; p0.05), but did not modify 45Ca influx to the cell. Insulin-induced relaxation was inhibited by indomethacin 10 microM, an antagonist of prostaglandin synthesis, and also by blockade of insulin receptors with 30 microM genistein. A short insulin preincubation (15 min) did not modify ET1 contractions. In rat tail artery, insulin preincubation (120 min) increased the force developed by ET1 (847 +/- 45 vs. 596 +/- 99 mgF/mgW in controls, n = 14) by stimulating TXA2 release and/or actions. In summary, the present results suggest that endothelial factors are involved in both the vasoconstrictor and vasodilator effects of insulin on rat vessels. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|