Secretion of immunoreactive -endorphin and GnRH by the pig uterus: effects of reproductive status and breed
| Autor: | S. Jiao, W. I. Li, C. L. Chen, Fuller W. Bazer |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Embryology
medicine.medical_specialty Swine Uterus Early pregnancy factor Peptide hormone Gonadotropin-Releasing Hormone Endocrinology Estrus Pregnancy Internal medicine medicine Animals Secretion Chromatography High Pressure Liquid Estrous cycle biology Reproduction beta-Endorphin Obstetrics and Gynecology Large white Cell Biology Breed Body Fluids medicine.anatomical_structure Reproductive Medicine In utero biology.protein Female |
| Zdroj: | Reproduction. 99:253-258 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/jrf.0.0990253 |
| Popis: | Immunoreactive beta-endorphin (ir-BEND) and GnRH (ir-GnRH) have been identified in the pig uterus. The study reported here examined (i) possible biochemical differences between ir-BEND and ir-GnRH present in pig uterine fluids and standard synthetic peptides, and (ii) the secretory profiles of uterine ir-BEND and ir-GnRH during the oestrous cycle and early pregnancy for two breeds of pig, Large White and the highly prolific Chinese Meishan. Reverse phase (RP)-HPLC analysis of concentrated uterine fluids indicated that the majority of ir-BEND eluted with a hydrophobicity similar to that of synthetic BEND 1-31 (BEND) and alpha-N-acetylated BEND 1-31 (Nac-BEND), with a ratio of BEND to Nac-BEND of approximately 1.8:1. The RP-HPLC profiles of ir-GnRH demonstrated a major peak coeluting with synthetic GnRH, along with a minor peak eluting at the void volume. Total content of ir-BEND (pg per uterus) was affected by the interaction of breed with day (P < 0.001), but was independent of reproductive status. In Large White gilts, uterine fluid content of ir-BEND was higher (P < 0.05) on days 10 and 11 than on days 8, 12 and 14; however, in Meishan gilts, ir-BEND decreased from day 8 to days 10 and 11, and remained low on days 12 and 14. Compared to Meishan gilts, Large White gilts had higher ir-BEND concentrations on day 10 (P < 0.01), day 11 (P < 0.0001), day 12 (P < 0.01) and day 14 (P < 0.02).(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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