A low level of lysophosphatidic acid in human gingival crevicular fluid from patients with periodontitis due to high soluble lysophospholipase activity: Its potential protective role on alveolar bone loss by periodontitis
| Autor: | Satoru Hashimura, Jun-ichi Kido, Risa Matsuda, Tamotsu Tanaka, Toshihiko Nagata, Hirokazu Matsui, Akira Tokumura, Miho Yokota, Toshihiko Tsutsumi, Yuji Inagaki, Mayumi Hidaka, Manami Inoue-Fujiwara |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Male Saliva medicine.medical_specialty Alveolar Bone Loss 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Internal medicine Lysophosphatidic acid medicine Choline Animals Humans Rats Wistar Interleukin 6 Periodontitis Molecular Biology Dental alveolus Cells Cultured Aged chemistry.chemical_classification biology Fatty acid Cell Biology Gingival Crevicular Fluid Middle Aged medicine.disease Rats 030104 developmental biology Endocrinology chemistry 030220 oncology & carcinogenesis biology.protein lipids (amino acids peptides and proteins) Female biological phenomena cell phenomena and immunity Lysophospholipids Lysophospholipase |
| Zdroj: | Biochimica et biophysica acta. Molecular and cell biology of lipids. 1865(7) |
| ISSN: | 1879-2618 |
| Popis: | We previously detected a submicromolar concentration of lysophosphatidic acid (LPA) in human saliva. Here, we compare LPA concentrations in human gingival crevicular fluid (GCF) from patients with periodontitis and healthy controls, and examine how the local LPA levels are regulated enzymatically. The concentrations of LPA and its precursor lysophospholipids in GCF was measured by liquid chromatography-tandem mass spectrometry. The LPA-producing and LPA-degrading enzymatic activities were measured by quantifying the liberated choline and free fatty acid, respectively. The concentration of LPA in GCF of periodontitis patients was lower than that of healthy controls, due to higher soluble lysophospholipase activity toward LPA. LPA was found to prevent survival of Sa3, a human gingival epithelium-derived tumor cell line, activate Sa3 through Ca2+ mobilization, and release interleukin 6 from Sa3 in vitro. Furthermore, local injection of LPA into the gingiva attenuated ligature-induced experimental alveolar bone loss induced by oral bacteria inoculation in a rat model of periodontitis in vivo. A high concentration of LPA in human GCF is necessary to maintain normal gingival epithelial integrity and function, protecting the progression of periodontitis. |
| Databáze: | OpenAIRE |
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