Disruption of the Bcchs3a chitin synthase gene in Botrytis cinerea is responsible for altered adhesion and overstimulation of host plant immunity
| Autor: | Caroline Kunz, Loïc Desquilbet, Delphine Arbelet, Thierry Fontaine, Marie-Christine Soulié, Pierrette Malfatti, Elizabeth Simond-Côte, Dominique Expert |
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| Přispěvatelé: | Interactions Plantes Pathogènes (IPP), Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National Agronomique Paris-Grignon (INA P-G), Aspergillus, Institut Pasteur [Paris] (IP), AgroParisTech, Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Indoles
MESH: Mutation Physiology Mutant Arabidopsis Virulence MESH: Thiazoles MESH: Botrytis MESH: Virulence Gene Expression Regulation Enzymologic Microbiology Fungal Proteins 03 medical and health sciences MESH: Plant Diseases Bcchs3a Gene Expression Regulation Fungal Camalexin Arabidopsis thaliana DEFENSE DES PLANTES MESH: Arabidopsis Mycelium [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology Plant Diseases 030304 developmental biology Botrytis cinerea Chitin Synthase chemistry.chemical_classification MESH: Indoles 0303 health sciences biology 030306 microbiology Phytoalexin MESH: Gene Expression Regulation Enzymologic fungi General Medicine Chitin synthase MESH: Chitin Synthase biology.organism_classification Thiazoles chemistry Mutation biology.protein MESH: Fungal Proteins Botrytis Agronomy and Crop Science MESH: Gene Expression Regulation Fungal |
| Zdroj: | Molecular Plant-Microbe Interactions Molecular Plant-Microbe Interactions, 2010, 23 (10), pp.1324-1334. ⟨10.1094/MPMI-02-10-0046⟩ Molecular Plant-Microbe Interactions, American Phytopathological Society, 2010, 23 (10), pp.1324-1334. ⟨10.1094/MPMI-02-10-0046⟩ |
| ISSN: | 0894-0282 |
| DOI: | 10.1094/MPMI-02-10-0046⟩ |
| Popis: | International audience; The fungal cell wall is a dynamic structure that protects the cell from different environmental stresses suggesting that wall synthesizing enzymes are of great importance for fungal virulence. Previously, we reported the isolation and characterization of a mutant in class III chitin synthase, Bcchs3a, in the phytopathogenic fungus Botrytis cinerea. We demonstrated that virulence of this mutant is severely impaired. Here, we describe the virulence phenotype of the cell-wall mutant Bcchs3a on the model plant Arabidopsis thaliana and analyze its virulence properties, using a variety of A. thaliana mutants. We found that mutant Bcchs3a is virulent on pad2 and pad3 mutant leaves defective in camalexin. Mutant Bcchs3a was not more susceptible towards camalexin than the wild-type strain but induced phytoalexin accumulation at the infection site on Col-0 plants. Moreover, this increase in camalexin was correlated with overexpression of the PAD3 gene observed as early as 18 h postinoculation. The infection process of the mutant mycelium was always delayed by 48 h, even on pad3 plants, probably because of lack of mycelium adhesion. No loss in virulence was found when Bcchs3a conidia were used as the inoculum source. Collectively, these data led us to assign a critical role to the BcCHS3a chitin synthase isoform, both in fungal virulence and plant defense response. |
| Databáze: | OpenAIRE |
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