A rapid and convenient LC/MS method for routine identification of methamphetamine/dimethylamphetamine and their metabolites in urine
| Autor: | Ka-Keung Chan, Ava Fung-man Li, Wing-Chi Cheng, Vincent King-Kuen Mok |
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| Rok vydání: | 2007 |
| Předmět: |
Analyte
Chromatography Chemistry Metabolite Amphetamine-Related Disorders Urine Mass spectrometry Sensitivity and Specificity Mass Spectrometry Methamphetamine Pathology and Forensic Medicine Substance Abuse Detection Amphetamine chemistry.chemical_compound Dimethylamphetamine Predictive Value of Tests Liquid chromatography–mass spectrometry medicine Ammonium formate Humans Solid phase extraction Law Chromatography Liquid medicine.drug |
| Zdroj: | Forensic Science International. 166:1-7 |
| ISSN: | 0379-0738 |
| Popis: | A rapid and sensitive LC/MS method was developed for the simultaneous analysis of N , N -dimethylamphetamine (DMA), N , N -dimethylamphetamine N -oxide (DMANO), methylamphetamine (MA) and amphetamine (A) in urine samples. Employing an Alltech C18 column for solid phase extraction followed by LC/MS analysis using an Alltech Platinum EPS C18 column with a mixture of ammonium formate (0.01 M, pH 3) and acetonitrile (77:23, v/v) as mobile phase at a flow rate of 0.2 mL/min, simultaneous identification and quantitation of A, MA, DMA and DMANO in urine can be achieved using a 5-min chromatographic run. The calibration ranges were 0.10–3.0 μg/mL for DMANO, 0.05–3.0 μg/mL for DMA and 0.05–5.0 μg/mL for both MA and A. The intra-, inter-day precision and accuracy for all analytes, spiked at three different concentrations in quality control samples, were in the ranges of 1.7–8.6, 4.1–10.0, −11.6 to 12.9%, respectively. The newly developed method was applied to the analysis of urine samples obtained from 118 suspected MA/DMA abusers, with the presence of MA confirmed in their urine samples under the drug-use surveillance program. Of these 118 samples, 43 were found to contain DMANO and 11 with both DMANO and DMA. |
| Databáze: | OpenAIRE |
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