Changes in surfactant-associated protein mRNA profile in growth-restricted fetal sheep
| Autor: | Jun Murotsuki, Kevin Inchley, Robert Gagnon, Fred Possmayer, Johnathan Langridge |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Pulmonary Surfactant-Associated Proteins Hydrocortisone Physiology Proteolipids Gestational Age Placental insufficiency Biology Dinoprostone Fetus Physiology (medical) Internal medicine Gene expression medicine Animals RNA Messenger Lung Pulmonary Surfactant-Associated Protein A Messenger RNA Fetal Growth Retardation Sheep Gestational age Pulmonary Surfactants Cell Biology DNA Organ Size medicine.disease Fetal Blood Endocrinology Gestation Glucocorticoid medicine.drug |
| Zdroj: | The American journal of physiology. 276(3) |
| ISSN: | 0002-9513 |
| Popis: | To test the hypothesis that chronic placental insufficiency resulting in fetal growth restriction causes an increase in fetal lung surfactant-associated protein (SP) gene expression, we embolized chronically catheterized fetal sheep ( n = 6) daily using nonradioactive microspheres in the abdominal aorta for 21 days (between 0.74 and 0.88 of gestation) until fetal arterial oxygen content was reduced by ∼40–50%. Control animals ( n = 7) received saline only. Basal fetal plasma cortisol concentration was monitored. At the end of the experiment, fetal lung tissues were collected, and ratios of tissue levels of SP-A, SP-B, and SP-C mRNA to 18S rRNA were determined by standard Northern blot analysis. Total DNA content of fetal lungs was reduced by 30% in the embolized group compared with control group ( P = 0.01). There was a 2.7-fold increase in fetal lung SP-A mRNA ( P< 0.05) and a 3.2-fold increase in SP-B mRNA ( P < 0.01) in the chronically embolized group compared with those in the control group. SP-A and SP-B mRNA tissue levels were highly correlated with the mean fetal plasma cortisol levels on days 20–21( r = 0.90, P < 0.01 for SP-A mRNA and r = 0.94, P < 0.01 for SP-B mRNA). SP-C mRNA tissue levels were not significantly affected by placental insufficiency. We conclude that fetal growth restriction due to placental insufficiency is associated with alterations in fetal lung SP, suggesting enhanced lung maturation that was highly dependent on the degree of increase in fetal plasma cortisol levels. |
| Databáze: | OpenAIRE |
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