Ethanol-Induced Reorganization of the Liquid-Ordered Phase: Enhancement of Cholesterol−Phospholipid Association
| Autor: | Jianbing Zhang, Honghua Cao, Bingwen Jing, Steven L. Regen |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Ethanol
1 2-Dipalmitoylphosphatidylcholine Stereochemistry Chemistry Liquid ordered phase Cholesterol Bilayer Lipid Bilayers technology industry and agriculture Phospholipid Biological membrane General Chemistry Biochemistry Article Catalysis Kinetics chemistry.chemical_compound Colloid and Surface Chemistry Membrane Phosphatidylcholines lipids (amino acids peptides and proteins) Lipid bilayer |
| Zdroj: | Journal of the American Chemical Society. 128:265-269 |
| ISSN: | 1520-5126 0002-7863 |
| Popis: | This paper records, what is believed to be, the first evidence for the reorganization of the liquid-ordered phase by ethanol. Specifically, ethanol has been found to significantly enhance sterol-phospholipid association in liquid-ordered bilayers derived from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) plus cholesterol, and also 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) plus cholesterol. The evidence for such reorganization comes from a series of nearest-neighbor recognition (NNR) experiments that have been carried out, where low concentrations of equilibrating lipid dimers (i.e., “reporter molecules”) have been used to detect changes in the phase composition of host membranes made from varying mixtures of DPPC/cholesterol, and also DSPC/cholesterol, in the presence and in the absence of ethanol. These findings have important biological implications, which are briefly discussed. |
| Databáze: | OpenAIRE |
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