Expression Pattern of α-Tubulin, Inversin and Its Target Dishevelled-1 and Morphology of Primary Cilia in Normal Human Kidney Development and Diseases
| Autor: | Anita Racetin, Snježana Mardešić, Danica Galešić-Ljubanović, Natalija Filipović, Ivana Bočina, Merica Glavina Durdov, Ivana Šolić, Mirna Saraga-Babić, Katarina Vukojević |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Pathology Nephrotic Syndrome Kidney / embryology α-tubulin Dishevelled Proteins 030232 urology & nephrology Kidney development Kidney lcsh:Chemistry MCDK 0302 clinical medicine Focal segmental glomerulosclerosis Dishevelled Proteins / metabolism Tubulin Multicystic Dysplastic Kidney / metabolism inversin Child lcsh:QH301-705.5 Spectroscopy chemistry.chemical_classification human kidney development Glomerulosclerosis Focal Segmental Cilium Kidney Tubules / metabolism Kidney / pathology General Medicine Cilia / metabolism Computer Science Applications Dishevelled Kidney Tubules CNF Glomerulosclerosis Focal Segmental / metabolism Signal Transduction medicine.medical_specialty DVL-1 FSGS Biology Article Catalysis Kidney morphogenesis Inorganic Chemistry 03 medical and health sciences Tubulin / metabolism medicine Humans Multicystic Dysplastic Kidney Cilia Physical and Theoretical Chemistry Molecular Biology Pathological Transcription Factors / metabolism urogenital system Gene Expression Profiling Organic Chemistry Infant medicine.disease 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 chemistry Gene Expression Regulation Cytoplasm Nephrotic Syndrome / metabolism Nephrotic syndrome Transcription Factors |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 7 International Journal of Molecular Sciences, Vol 22, Iss 3500, p 3500 (2021) |
| Popis: | The spatiotemporal expression of α-tubulin, inversin and dishevelled-1 (DVL-1) proteins associated with the Wnt-signaling pathway, and primary cilia morphology were analyzed in developing kidneys (14th–38th developmental weeks), healthy postnatal (1.5- and 7-years old) and pathologically changed human kidneys, including multicystic dysplastic kidneys (MCDK), focal segmental glomerulosclerosis (FSGS) and nephrotic syndrome of the Finnish type (CNF). The analysis was performed by double immunofluorescence, electron microscopy, semiquantitative and statistical methods. Cytoplasmic co-expression of α-tubulin, inversin and DVL-1 was observed in the proximal convoluted tubules (pct), distal convoluted tubules (dct) and glomeruli (g) of analyzed tissues. During kidney development, the overall expression of α-tubulin, inversin and DVL-1 decreased, while in the postnatal period slightly increased. The highest expressions of α-tubulin and inversin characterized dct and g, while high DVL-1 characterized pct. α-tubulin, inversin and DVL-1 expression pattern in MCDK, FSGS and CNF kidneys significantly differed from the healthy control. Compared to healthy kidneys, pathologically changed kidneys had dysmorphic primary cilia. Different expression dynamics of α-tubulin, inversin and DVL-1 during kidney development could indicate that switch between the canonical and noncanonical Wnt-signaling is essential for normal kidney morphogenesis. In contrast, their disturbed expression in pathological kidneys might be associated with abnormal primary cilia, leading to chronic kidney diseases. |
| Databáze: | OpenAIRE |
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