Inhibition of topoisomerase IIα: novel function of wedelolactone
| Autor: | Lucia Knopfová, Miroslav Fojta, Diana Pinheiro, Filip Trčka, Karel Souček, Petr Beneš, Alice Nemajerova, Jan Šmarda |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Cancer Research
DNA damage Cell Survival Topoisomerase Inhibitors Immunoblotting Antineoplastic Agents Apoptosis Breast Neoplasms Enzyme-Linked Immunosorbent Assay Cell Growth Processes 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Antigens Neoplasm Coumarins Cell Line Tumor Humans Transcription factor 030304 developmental biology 0303 health sciences biology Topoisomerase Cell Cycle Wedelolactone 3. Good health Cell biology Androgen receptor DNA-Binding Proteins DNA Topoisomerases Type II Oncology chemistry 030220 oncology & carcinogenesis Cancer cell biology.protein Cancer research Female Signal transduction DNA Damage Signal Transduction |
| Zdroj: | Cancer letters. 303(1) |
| ISSN: | 1872-7980 |
| Popis: | The naturally occurring coumestan wedelolactone has been previously shown to reduce growth of various cancer cells. So far, the growth-suppressing effect of wedelolactone has been attributed to the inhibition of the NFκB transcription factor and/or androgen receptors. We found that wedelolactone suppressed growth and induced apoptosis of androgen receptor-negative MDA-MB-231 breast cancer cells at concentrations that did not inhibit the NFκB activity. The cells responded to wedelolactone by the S and G2/M phase cell cycle arrest and induction of the DNA damage signaling. Wedelolactone interacted with dsDNA and inhibited the activity of DNA topoisomerase IIα. We conclude that wedelolactone can act as growth suppressor independently of NFκB and androgen receptors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect