Radioprotective potential of Lagenaria siceraria extract against radiation-induced gastrointestinal injury
| Autor: | Dhara Sharma, Harish Chandra Goel, Sonal Chauhan |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
G2 Phase
Male 0301 basic medicine Gastrointestinal Diseases Physiology Lagenidium Endocrinology Diabetes and Metabolism Radiation-Protective Agents Gastrointestinal Injury Radiation induced Tight Junctions Mice Random Allocation 03 medical and health sciences chemistry.chemical_compound Cucurbita pepo 0302 clinical medicine Cucurbita In vivo Cell Line Tumor Physiology (medical) Botany Animals Irradiation Food science Intestinal Mucosa Nutrition and Dietetics Microvilli biology Plant Extracts Lagenaria General Medicine Glutathione biology.organism_classification Survival Analysis Radiation Effects Radiation Injuries Experimental Prebiotics 030104 developmental biology chemistry Novel agents Fruit 030220 oncology & carcinogenesis Luffa DNA Damage |
| Zdroj: | Applied Physiology, Nutrition, and Metabolism. 41:1248-1254 |
| ISSN: | 1715-5320 1715-5312 |
| DOI: | 10.1139/apnm-2016-0136 |
| Popis: | The cucurbits (prebiotics) were investigated as novel agents for radio-modification against gastrointestinal injury. The cell-cycle fractions and DNA damage were monitored in HCT-15 cells. A cucurbit extract was added to culture medium 2 h before irradiation (6 Gy) and was substituted by fresh medium at 4 h post-irradiation. The whole extract of the fruits of Lagenaria siceraria, Luffa cylindrica, or Cucurbita pepo extract enhanced G2 fractions (42%, 34%, and 37%, respectively) as compared with control (20%) and irradiated control (31%). With cucurbits, the comet tail length remained shorter (L. siceraria, 28 μm; L. cylindrica, 34.2 μm; C. pepo, 36.75 μm) than irradiated control (41.75 μm). For in vivo studies, L. siceraria extract (2 mg/kg body weight) was administered orally to mice at 2 h before and 4 and 24 h after whole-body irradiation (10 Gy). L. siceraria treatment restored the glutathione contents to 48.8 μmol/gm as compared with control (27.6 μmol/gm) and irradiated control (19.6 μmol/gm). Irradiation reduced the villi height from 379 to 350 μm and width from 54 to 27 μm. L. siceraria administration countered the radiation effects (length, 366 μm; width, 30 μm, respectively) and improved the villi morphology and tight junction integrity. This study reveals the therapeutic potential of cucurbits against radiation-induced gastrointestinal injury. |
| Databáze: | OpenAIRE |
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