Molecular characterization of Clostridioides difficile ribotype 027 in a major Chinese hospital
| Autor: | Yong Wang, Cong-hui Wang, Chun-hong Shao, Chun-mei Liu, Yan Jin, Yu-xia Man, Yong-xing Lei, Yuanyuan Bai, Ren-feng Zhang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) China Genotype 030106 microbiology Antimicrobial susceptibility Microbial Sensitivity Tests Biology Antimicrobial resistance Microbiology Ribotyping 03 medical and health sciences 0302 clinical medicine Antibiotic resistance Drug Resistance Bacterial Immunology and Allergy Whole genome sequence Humans National level 030212 general & internal medicine Phylogeny Retrospective Studies Whole genome sequencing PCR RT027 GeneXpert MTB/RIF General Immunology and Microbiology Clostridioides difficile General Medicine Pcr ribotyping Virology QR1-502 Hospitals Anti-Bacterial Agents Infectious Diseases Clostridium Infections Multilocus sequence typing Clostridioides Genome Bacterial Multilocus Sequence Typing |
| Zdroj: | Journal of Microbiology, Immunology and Infection, Vol 54, Iss 6, Pp 1179-1183 (2021) |
| ISSN: | 1995-9133 |
| Popis: | Background The rapid spread of C. difficile 027 has become one of the leading threats of healthcare-associated infections wordwild. However, C. difficile 027 infections have rarely been reported in China. The objective of this study was to strengthen the understanding of the molecular characterizations of C. difficile 027 in China. Methods In this study, stool specimens from 176 suspected CDI cases were collected from 1 Jan 2018 to 30 Jun 2019. These specimens were measured by GeneXpert test and C.difficile colonies were identified and analyzed. Results There were five samples positive for tcdA, tcdB, binary toxin genes and had deletions in tcdC gene. These five Clostridioides difficile isolates belonged to ST1 and confirmed as Clostridioides difficile 027 strains by PCR ribotyping. Through using whole genome sequencing, , we found that these five strains were closely clustered into the same predominant evolutionary branch and were highly similar to C. difficile 027 strain R20291. Antimicrobial susceptibility testing result showed they were highly resistant to fluoroquinolones. Conclusions In Our study, five C. difficile 027 isolates were identified and characterized using MLST, PCR ribotyping and whole genome sequencing. We proposed that C. difficile 027 infections are probably neglected in China. Further epidemiological studies across the country together with the introduction of routine diagnostic testing and multi-center or national level surveillance are needed to ascertain the size of this potentially significant problem. |
| Databáze: | OpenAIRE |
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