The Human Enhancer Blocker CTC-binding Factor Interacts with the Transcription Factor Kaiso
| Autor: | Curtis L. Nordgaard, Eric Gilson, Roberto Pérez-Torrado, Pierre-Antoine Defossez, Kevin F. Kelly, Frédérique Magdinier, Guillaume J. Filion, Juliet M. Daniel, Hervé Menoni |
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| Přispěvatelé: | Centre épigénétique et destin cellulaire (EDC), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), McMaster University [Hamilton, Ontario], Dynamique nucléaire et plasticité du génome (DNPG), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie [Paris]-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie Moléculaire de la Cellule (LBMC), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Magdinier, Frederique, Centre épigénétique et destin cellulaire (EDC (UMR_7216)), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7) |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
CCCTC-Binding Factor
Transcription Genetic Insulator (genetics) Biochemistry Genome 0302 clinical medicine 0303 health sciences CTC-BINDING FACTOR EXPRESSION DU GENE Chemistry adn Antibodies Monoclonal Zinc Fingers Globins DNA-Binding Proteins Enhancer Elements Genetic CpG site Spectrophotometry 030220 oncology & carcinogenesis [SDV.IMM]Life Sciences [q-bio]/Immunology Protein Binding Chromatin Immunoprecipitation DNA Complementary [SDV.IMM] Life Sciences [q-bio]/Immunology Ultraviolet Rays Immunoblotting Molecular Sequence Data 03 medical and health sciences Two-Hybrid System Techniques [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Humans Immunoprecipitation [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Binding site Enhancer Molecular Biology Transcription factor 030304 developmental biology Binding Sites Base Sequence Genome Human génome gène Cell Biology DNA Methylation Molecular biology Ctcf binding Protein Structure Tertiary Repressor Proteins CTCF CpG Islands facteur de transcription HeLa Cells Transcription Factors |
| Zdroj: | Journal of Biological Chemistry Journal of Biological Chemistry, 2005, 280 (52), pp.43017-43023. ⟨10.1074/jbc.M510802200⟩ Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2005, 280 (52), pp.43017-43023. ⟨10.1074/jbc.M510802200⟩ Journal of Biological Chemistry 52 (280), 43017-43023. (2005) |
| ISSN: | 0021-9258 1083-351X |
| DOI: | 10.1074/jbc.M510802200⟩ |
| Popis: | International audience; CTC-binding factor (CTCF) is a DNA-binding protein of vertebrates that plays essential roles in regulating genome activity through its capacity to act as an enhancer blocker. We performed a yeast two-hybrid screen to identify protein partners of CTCF that could regulate its activity. Using full-length CTCF as bait we recovered Kaiso, a POZ-zinc finger transcription factor, as a specific binding partner. The interaction occurs through a C-terminal region of CTCF and the POZ domain of Kaiso. CTCF and Kaiso are co-expressed in many tissues, and CTCF was specifically co-immu-noprecipitated by several Kaiso monoclonal antibodies from nuclear lysates. Kaiso is a bimodal transcription factor that recognizes methylated CpG dinucleotides or a conserved unmethylated sequence (TNGCAGGA, the Kaiso binding site). We identified one consensus unmethylated Kaiso binding site in close proximity to the CTCF binding site in the human 5-globin insulator. We found, in an insulation assay, that the presence of this Kaiso binding site reduced the enhancer-blocking activity of CTCF. These data suggest that the Kaiso-CTCF interaction negatively regulates CTCF insulator activity. |
| Databáze: | OpenAIRE |
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