Rapid Development of KASP Markers for Disease Resistance Genes Using Pooled Whole-Genome Resequencing
| Autor: | Colum Kennedy, Stephen Byrne, Dan Milbourne, Denis Griffin, Fergus Meade, Francesca Mesiti |
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| Rok vydání: | 2019 |
| Předmět: |
2. Zero hunger
0106 biological sciences fungi food and beverages Single-nucleotide polymorphism 04 agricultural and veterinary sciences Computational biology Biology Plant disease resistance 01 natural sciences Phenotype 040103 agronomy & agriculture 0401 agriculture forestry and fisheries SNP Identification (biology) Agronomy and Crop Science Gene Genotyping Selection (genetic algorithm) 010606 plant biology & botany Food Science |
| Zdroj: | Potato Research |
| ISSN: | 1871-4528 0014-3065 |
| DOI: | 10.1007/s11540-019-09428-x |
| Popis: | Marker-assisted selection (MAS) is becoming increasingly common in commercial potato breeding programmes and can provide information to breeders on potential resistance to diseases and pests early in the breeding cycle when phenotypic screens are impractical. In recent years, a wealth of information on molecular markers for genes involved in resistance to biotic stresses of potato has been revealed. However, markers come in many different forms and ideally a breeding programme would routinely perform annual genotyping on a single unified platform. In this study, we present an approach to consolidate various types of molecular markers to a single SNP-based platform using whole-genome sequencing of carefully constructed pools of DNA using, as examples, molecular markers for the H1 nematode and R2 blight resistance genes. The approach led to the identification of a number of candidate nucleotide polymorphisms which allowed successful design and deployment of kompetitive allele-specific PCR (KASP) assays. We further show how the sequence data generated can be used to aid design of KASP assays for diagnostic SNPs for other traits mined from the literature. |
| Databáze: | OpenAIRE |
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