Blood thyroglobulin and TSH receptor mRNA detection by RT-PCR in the follow-up of differentiated thyroid cancer patients
| Autor: | C. Belzarena, B. Alvarez, Graciela Lago, G. Manrique, L. Torosian, R. Roca |
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| Rok vydání: | 2010 |
| Předmět: |
Male
endocrine system medicine.medical_specialty medicine.medical_treatment Levothyroxine Sensitivity and Specificity Thyroglobulin Iodine Radioisotopes Predictive Value of Tests Internal medicine Adenocarcinoma Follicular Biomarkers Tumor medicine Carcinoma Humans False Positive Reactions Radiology Nuclear Medicine and imaging RNA Messenger RNA Neoplasm Thyroid Neoplasms Receptor Thyroid cancer Reverse Transcriptase Polymerase Chain Reaction business.industry Thyroidectomy Cell Differentiation Receptors Thyrotropin Neoplastic Cells Circulating medicine.disease Combined Modality Therapy Carcinoma Papillary Real-time polymerase chain reaction Endocrinology Adenocarcinoma Female Radiopharmaceuticals business Follow-Up Studies medicine.drug |
| Zdroj: | Revista Española de Medicina Nuclear. 29:109-113 |
| ISSN: | 0212-6982 |
| DOI: | 10.1016/j.remn.2009.12.010 |
| Popis: | Objective During the last years several groups have used the technique of RT-PCR for the detection of circulating thyroid cells, through the amplification of thyroglobulin (Tg) and TSH receptor(TSH-R) mRNA; however the published results are controversial. In this study we investigated the utility for the detection of Tg and TSH-R mRNA by RT-PCR in patients with differentiated thyroid cancer (DTC) during treatment with levothyroxine. Subjects and methods We investigated the expression of Tg and TSH-R mRNA by single and nested RT-PCR in the blood of 3 groups of subjects: (A) 34 patients with DTC and no evidence of disease, (B) 8 patients with DTC and evidence of local or distant metastasis and (C) 13 normal subjects. Expression levels of Tg mRNA were also analysed by comparative semi-quantitative RT-PCR. Results Tg and TSH-R mRNA signals were detected in all subjects (patients with DTC with and without evidence of disease and in normal subjects) by single or nested RT-PCR. By semi-quantitative RT-PCR and densitometric analysis of PCR products, mean levels of circulating Tg mRNA of the 3 groups were: Group A 0.182±0.107, Group B 0.329±0.298 and Group C 0.305±0.217. Conclusions Single or nested RT-PCR for Tg and TSH-R mRNA is not a suitable tool in the follow-up of patients with DTC. Lower levels of Tg mRNA in patients with DTC without evidence of disease, although not significant, may indicate that small numbers of thyroid cells may be normally present in the circulation or may represent an ectopic transcriptionof messengers from blood cells |
| Databáze: | OpenAIRE |
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