Tetrandrine Inhibits Epithelial-Mesenchymal Transition in IL-6-Induced HCT116 Human Colorectal Cancer Cells

Autor:	Shih-Chang Tsai, Wei-Chei Wu, Jai-Sing Yang
Rok vydání:	2021
Předmět:	IL-6 epithelial-mesenchymal transition colorectal cancer Cell migration tetrandrine medicine.disease OncoTargets and Therapy Metastasis Blot Tetrandrine chemistry.chemical_compound Oncology chemistry Cancer cell Cancer research medicine Pharmacology (medical) MTT assay Epithelial–mesenchymal transition Wound healing Original Research
Zdroj:	OncoTargets and therapy
ISSN:	1178-6930
Popis:	Shih-Chang Tsai,1 Wei-Chei Wu,1 Jai-Sing Yang2 1Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, Republic of China; 2Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan, Republic of ChinaCorrespondence: Shih-Chang TsaiDepartment of Biological Science and Technology, China Medical University, 100 Sec. 1, Jing-Mao Road, Taichung, 406040, Taiwan, Republic of ChinaTel +886 4-2205-3366 ext 2518Fax +886 4-2299-4787Email sctsai@mail.cmu.edu.twIntroduction: Patients with colorectal cancer (CRC) often develop distant metastases, which significantly reduces the 5-year survival rate. Epithelial-mesenchymal transition (EMT) is a crucial process for the invasion and metastasis of cancer cells. Tetrandrine has been reported to inhibit the viability and EMT of CRC cells; however, to the best of our knowledge, the molecular mechanism remains undetermined.Methods: The MTT assay was used to determine HCT116 cell viability. Wound healing and Transwell assays were used to determine that cell migration and invasion, respectively. Western blotting analysis was performed to detect the expression of migration-related genes. Four different lengths of the E-cadherin gene promoter were constructed and cloned into pGL3 reporter plasmids to evaluate E-cadherin gene promoter activity.Results: The results of the MTT assay revealed that tetrandrine inhibited HCT116 cell viability, with an IC50 value of 7.2 μM following 24 h of treatment. Tetrandrine inhibited IL-6-induced cell migration and invasion, respectively. Tetrandrine regulates the expression of migration-related genes in IL-6-stimulated HCT116 cells. Tetrandrine significantly downregulated the expression and enzyme activity of MMP-2 in IL-6-stimulated HCT116 cells. In addition, tetrandrine restored E-cadherin gene promoter activity.Conclusion: The findings of the present study suggested that tetrandrine may inhibit EMT in IL-6-stimulated HCT116 cells; therefore, it may represent a potential drug for CRC.Keywords: tetrandrine, IL-6, epithelial-mesenchymal transition, colorectal cancer
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0dd08f1aaf44058262d5685416b9247 https://doi.org/10.2147/ott.s324552 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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