PCR-based molecular discrimination betweenMaritrema eroliaeandProbolocoryphe uca(Digenea: Microphallidae) in Kuwait Bay
| Autor: | Suzanne A. Al-Bustan, Wafa Y. Al-Kandari, Majed A. Alnaqeeb, Asha Merine Isaac |
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| Rok vydání: | 2013 |
| Předmět: |
Zoology
Biology Polymerase Chain Reaction Digenea law.invention law DNA Ribosomal Spacer Animals Ribosomal DNA Polymerase chain reaction DNA Primers Larva General Medicine DNA Helminth biology.organism_classification Crustacean Fishery Restriction enzyme Kuwait Animal Science and Zoology Parasitology Trematoda Restriction fragment length polymorphism Bay Polymorphism Restriction Fragment Length |
| Zdroj: | Journal of Helminthology. 88:177-182 |
| ISSN: | 1475-2697 0022-149X |
| DOI: | 10.1017/s0022149x12000892 |
| Popis: | Microphallid trematodes are common parasites in marine snails and crustacean hosts at Kuwait Bay. The larval stages of two microphallids,Maritrema eroliaeandProbolocoryphe uca, are difficult to differentiate morphologically. In this study, two PCR-based techniques were established for quick and accurate discrimination between the larval stages of the two microphallid species, employing restriction fragment length polymorphism (PCR-RFLP) and species-specific primers. Both techniques utilized nucleotide differences in the second internal transcribed region (ITS2) of the ribosomal DNA (rDNA) in the two species. For the PCR-RFLP technique, restriction enzymeAvaII was selected and it generated different restriction profiles among the two microphallids. In addition, species-specific primers were prepared for each microphallid species that amplified distinctive fragments. Both techniques showed that the larval stages of the two microphallid species can be identified accurately. However, direct PCR amplification using species-specific primers was more advantageous than the PCR-RFLP technique since it allowed rapid and specific discrimination between the two species. This technique provides a useful tool that can be used in future studies for the study of the distribution of microphallid species and their definitive hosts at different localities of Kuwait Bay. |
| Databáze: | OpenAIRE |
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