Light-enhanced genetic toxicity of crystal violet
| Autor: | David E. Levin, Thomas J. Lovely, Ed Klekowski |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Salmonella typhimurium
Salmonella food.ingredient DNA Repair Light Photochemistry medicine.disease_cause Ames test Agar plate chemistry.chemical_compound food Escherichia coli medicine Animals Agar Crystal violet Chromatography Mutagenicity Tests Methyl violet Rats Inbred Strains General Medicine Rats Surface coating chemistry Inactivation Metabolic Toxicity Gentian Violet Mutagens |
| Zdroj: | Mutation Research Letters. 103:283-288 |
| ISSN: | 0165-7992 |
| DOI: | 10.1016/0165-7992(82)90055-0 |
| Popis: | Crystal violet has been used not only in experimental biology to study a variety of biological and biochemical processes ]3], but has had numerous industrial uses including dying and surface coating of paper, and preparation of ball-point-pen inks and typewriter ribbons [4, 10]. It is also used medicinally as an antiseptic in the treatment of burns and pubic lice [4] and in gum and toe therapy [7] and commercially in hair dyes. A previous study of the mutagenicity of hair-dye components reported that crystal violet (alias methyl violet, or gentian violet) was not mutagenic for either Saccharomyces cerevisiae strain XV185-14C, or for any of 5 strains of Salmonella typhimurium [10]. A more recent study confirmed these negative results for 4 strains of Salmonella, showing only toxicity, but reported genetic toxicity with the Rosenkranz E. coli polymerase Asystem [2]. The latter study also reported that in the presence of rat-liver microsomes, the genetic toxicity of crystal violet was eliminated in the Ames assay and somewhat reduced in the Rosenkranz assay. The authors suggested that this action was due to metabolic inactivation of crystal violet by the liver detoxification system. The present study concerns the light-enhanced genetic toxicity of crystal violet and the effect of rat-liver $9 fraction on its genetic toxicity in the Rosenkranz assay and in 3 strains of Salmonella. The E. coli polymerase Aassay was used to detect repairable DNA damage. The wild-type strain (W3110) and its polymerase A deficient mutant (p3478) were kindly provided by Dr. H.S. Rosenkranz. The assay was conducted as described by Slater et al. [ 11 ]. Repairable DNA damage was detected by dispensing 0.1-ml aliquots of a log-phase culture, with 0.5 ml of $9, if indicated (0.08 ml/ml mix) into 2.0 ml of molten top agar. The mixture was then poured onto the surface of an agar plate. Both the top agar and the bottom agar contained HA medium supplemented with 5.0 ~g/ml of thymine [9]. After solidification of the top agar, a sterile 6-mm filter paper disc impregnated with 10/~1 of a known concentration of the test agent was |
| Databáze: | OpenAIRE |
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