A novel 'dual-potential' ratiometric electrochemiluminescence DNA sensor based on enhancing and quenching effect by G-quadruplex / hemin and Au-Luminol bifunctional nanoparticles
| Autor: | Xiaoquan Lu, Xiaohui Zhang, Yanfeng Wang, Guofan Wu, Yaxuan Qian, Duoliang Shan, Ru Fan, Huan Wang, Li Linfang |
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| Rok vydání: | 2018 |
| Předmět: |
endocrine system
Biomedical Engineering Biophysics Deoxyribozyme Metal Nanoparticles Nanoparticle chemical and pharmacologic phenomena Biosensing Techniques 02 engineering and technology 010402 general chemistry Photochemistry G-quadruplex 01 natural sciences Luminol chemistry.chemical_compound Limit of Detection Quantum Dots Electrochemistry Humans Electrochemiluminescence heterocyclic compounds Bifunctional Peroxidase Layer by layer DNA DNA Catalytic General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences G-Quadruplexes chemistry Luminescent Measurements Hemin Gold 0210 nano-technology Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 106:64-70 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2018.01.052 |
| Popis: | An ultrasensitive and stable "dual-potential" ratiometric electrochemiluminescence (ECL) sensor is reported for specific DNA, the femtomolar detection limit (0.12 fM, S/N = 3) and high selectivity insure its potential applications in cancer biomarkers searching or monitoring. The excellent performance of the sensor comes from simultaneously fabricated layer by layer structure "target DNA + Hemin / Au-Luminol NPs / DNA* / sl DNA / TGA / QDs / MWNTs / GCE" mode which was based on the enhancing effect of luminol by G-quadruplex / hemin and Au nanoparticles and the quenching effect of CdSe/ZnS by G-quadruplex / hemin. (i) DNA-SH could combine with Au-Luminol NPs via S-Au bond to solve the problem of poor solubility and weak ECL intensity of luminol in neutral medium. (ii) Target DNA and Hemin formed the G-quadruplex / hemin peroxidase mimicking DNAzyme could enhance the ECL of luminol and quench the ECL of CdSe/ZnS simultaneously. (iii) DNA* was employed to increase a certain distance between CdSe/ZnS and Au-Luminol for enhancing the CdSe/ZnS QDs initial ECL intensity. The dual-potential ratiometric mode lower the influence of background and side reaction of the ECL sensor which were the most important factors in trace sensing. |
| Databáze: | OpenAIRE |
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