Enhanced Tolerance against a Fungal Pathogen and Insect Resistance in Transgenic Tobacco Plants Overexpressing an Endochitinase Gene from Serratia marcescens
| Autor: | Víctor Manuel Hernández-Velázquez, Samantha Sarai Navarro-González, Laura Lina-Garcia, Gerardo Corzo, Guadalupe Peña-Chora, Ramón Suárez-Rodríguez, Abigail Roldán-Salgado, José Augusto Ramírez-Trujillo, Paul Gaytán |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Nicotiana tabacum Transgene viruses Genetically modified crops Spodoptera transgenic plants 01 natural sciences Catalysis Microbiology Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences Physical and Theoretical Chemistry Molecular Biology lcsh:QH301-705.5 Spectroscopy Botrytis cinerea biology Serratia marcescens Organic Chemistry fungi antifungal activity food and beverages General Medicine biology.organism_classification bacterial chitinase Computer Science Applications Transformation (genetics) 030104 developmental biology Spodoptera frugiperda lcsh:Biology (General) lcsh:QD1-999 Chitinase biology.protein 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences, Vol 20, Iss 14, p 3482 (2019) International Journal of Molecular Sciences Volume 20 Issue 14 |
| ISSN: | 1422-0067 |
| Popis: | In this study we cloned a chitinase gene (SmchiC), from Serratia marcescens isolated from the corpse of a Diatraea magnifactella lepidopteran, which is an important sugarcane pest. The chitinase gene SmchiC amplified from the S. marcescens genome was cloned into the transformation vector p2X35SChiC and used to transform tobacco (Nicotiana tabacum L. cv Petit Havana SR1). The resistance of these transgenic plants to the necrotrophic fungus Botrytis cinerea and to the pest Spodoptera frugiperda was evaluated: both the activity of chitinase as well as the resistance against B. cinerea and S. frugiperda was significantly higher in transgenic plants compared to the wild-type. |
| Databáze: | OpenAIRE |
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