Combined effect of IL-17 and blockade of nitric oxide biosynthesis on haematopoiesis in mice
| Autor: | Aleksandra Krstić, Ivana Okic, Gordana Jovčić, Pavle Milenković, S. Mojsilović, Jelena Kocic, J. F. Santibanez, Diana Bugarski |
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| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty bone marrow Physiology Reticulocytosis Bone Marrow Cells Biology Nitric Oxide Nitric oxide 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Internal medicine haematopoietic progenitors medicine Animals Cell Lineage Progenitor cell Enzyme Inhibitors 030304 developmental biology 0303 health sciences Interleukin-17 Hematopoietic Stem Cells NOS Hematopoiesis Nitric oxide synthase Haematopoiesis IL-17 Endocrinology medicine.anatomical_structure NG-Nitroarginine Methyl Ester chemistry Immunology biology.protein Mice Inbred CBA Erythropoiesis spleen Female Myelopoiesis Bone marrow medicine.symptom Nitric Oxide Synthase Spleen 030215 immunology |
| Zdroj: | Acta Physiologica |
| ISSN: | 1748-1716 |
| Popis: | Aim: The study was undertaken to extend our investigation concerning both the in vivo activity of interleukin (IL)-17 and the specific role of nitric oxide (NO) in IL-17-induced effects in the process of haematopoiesis. Methods: CBA mice were simultaneously treated with IL-17 and/or nitric oxide synthase (NOS) inhibitor, l-NAME, for 5 days and changes within various haematopoietic cell lineages in bone marrow, spleen and peripheral blood were analysed. Results: Findings showed that administration of both IL-17 and l-NAME stimulated increase in net haematopoiesis in normal mice. IL-17-enhanced myelopoiesis was characterized by stimulation of both femoral and splenic haematopoietic progenitor cells and morphologically recognizable granulocytes. Additionally, IL-17 induced alterations in the frequency of erythroid progenitor cells in both bone marrow and spleen, accompanied with their mobilization to the peripheral blood. As a consequence of these changes in the erythroid cell compartments, significant reticulocytosis was observed, which evidenced that in IL-17-treated mice effective erythropoiesis occurred. Exposure of mice to NOS inhibitor also increased the number of both granulocyte-macrophage and erythroid progenitors in bone marrow and spleens, and these alterations were followed by the mobilization of erythroid progenitors and elevated content of reticulocytes in peripheral blood. The specific role of NO in IL-17-induced haematopoiesis was demonstrated only in the IL-17-reducing effect on bone marrow late stage erythroid progenitors, CFU-E. Conclusion: The results demonstrated the involvement of both IL-17 and NO in the regulation of haematopoietic cell activity in various haematopoietic compartments. They further suggest that IL-17 effects are differentially mediated depending on the haematopoietic microenvironments. |
| Databáze: | OpenAIRE |
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