Molinate biodegradation in soils
| Autor: | Olga C. Nunes, Célia M. Manaia, Ana R. Lopes, Anthony S. Danko |
|---|---|
| Přispěvatelé: | Faculdade de Engenharia |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
DNA
Bacterial Bioaugmentation Molecular Sequence Data Heterotroph Natural sciences Biological sciences DNA Ribosomal Applied Microbiology and Biotechnology Paddy field soil Bioremediation Thiocarbamates RNA Ribosomal 16S Cluster Analysis Soil Pollutants Biological sciences [Natural sciences] Biotransformation Phylogeny Soil Microbiology Bacteria Chemistry Ecology Molinate Azepines Sequence Analysis DNA General Medicine Contamination Biodegradation 16S ribosomal RNA Biota Ciências Naturais Ciências biológicas Environmental chemistry Soil water Bacterial community Ciências biológicas [Ciências exactas e naturais] Microcosm Natural attenuation Biotechnology |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-012-4096-y |
| Popis: | The aims of the present study were to assess the potential of natural attenuation or bioaugmentation to reduce soil molinate contamination in paddy field soils and the impact of these bioremediation strategies on the composition of soil indigenous microbiota. A molinate mineralizing culture (mixed culture DC) was used as inoculum in the bioaugmentation assays. Significantly higher removal of molinate was observed in bioaugmentation than in natural attenuation microcosms (63 and 39 %, respectively) after 42 days of incubation at 22 A degrees C. In the bioaugmentation assays, the impact of Gulosibacter molinativorax ON4(T) on molinate depletion was observed since the gene encoding the enzyme responsible for the initial molinate breakdown (harboured by that actinobacterium) was only detected in inoculated microcosms. Nevertheless, the exogenous mixed culture DC did not overgrow as the heterotrophic counts of the bioaugmentation microcosms were not significantly different from those of natural attenuation and controls. Moreover, the actinobacterial clone libraries generated from the bioaugmentation microcosms did not include any 16S rRNA gene sequences with significant similarity to that of G. molinativorax ON4(T). The multivariate analysis of the 16S rRNA DGGE patterns of the soil microcosm suggested that the activity of mixed culture DC did not affect the soil bacterial community structure since the DGGE patterns of the bioaugmentation microcosms clustered with those of natural attenuation and controls. Although both bioremediation approaches removed molinate without indigenous microbiota perturbation, the results suggested that bioaugmentation with mixed culture DC was more effective to treat soils contaminated with molinate. |
| Databáze: | OpenAIRE |
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