The imbalance of Th17/Treg via STAT3 activation modulates cognitive impairment in P. gingivalis LPS-induced periodontitis mice
Autor: | Xuan Zhang, Xu Zhang, Che Qiu, Zhongchen Song, Zhiyan He, Huanyu Zhang, Hui Shen, Wei Zhou |
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Rok vydání: | 2021 |
Předmět: |
Lipopolysaccharides
STAT3 Transcription Factor Immunology Gingiva Spatial Learning Treg cell T-Lymphocytes Regulatory Stat3 Signaling Pathway Flow cytometry Mice Memory medicine Alveolar Process Immunology and Allergy Animals Cognitive Dysfunction Bone Resorption Cognitive impairment STAT3 Periodontitis Porphyromonas gingivalis medicine.diagnostic_test biology Cell Biology Acquired immune system biology.organism_classification medicine.disease Astrocytes biology.protein Cytokines Th17 Cells Microglia Signal Transduction |
Zdroj: | Journal of leukocyte biologyREFERENCES. 110(3) |
ISSN: | 1938-3673 |
Popis: | Periodontitis is one of the most common oral diseases worldwide, and it is associated with various systemic diseases, including cognitive diseases. STAT3 regulates the inflammatory cascade and influences adaptive immunity by modulating Th17/Treg cell differentiation. In this study, we aimed to explore the effect of adaptive immunity inside and outside the brain on the association between periodontitis and cognitive impairment and understand the role of the STAT3 signaling pathway. We established Porphyromonas gingivalis LPS-induced periodontitis mice models by injecting P. gingivalis LPS into the gingival sulcus of mice. Behavioral tests showed that learning and memory abilities were impaired. The flow cytometry data showed an imbalance in the Th17/Treg ratio in the blood and brain samples of the mice. The expression of Th17-related cytokines (IL-1β, IL-17A, IL-21, and IL-22) increased, whereas that of Treg-related cytokines (IL-2 and IL-10) decreased in both the blood and the brain. The level of LPS increased and the STAT3 signaling pathway was activated during this process. These effects were reversed by C188-9, a STAT3 inhibitor. In conclusion, P. gingivalis LPS-induced periodontitis may promote the occurrence and progression of cognitive impairment by modulating the Th17/Treg balance inside and outside the brain. The STAT3 signaling pathway may have immunoregulatory effects on the mouth-to-brain axis. |
Databáze: | OpenAIRE |
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