In Vivo Use of 1D and 2D 1H NMR to Examine the Glycosylation of Scopoletin in Duboisia myoporoides Cell Suspensions
| Autor: | Ophélie Fliniaux, Dominique Cailleu, Albrecht Roscher, François Mesnard |
|---|---|
| Přispěvatelé: | Biologie des Plantes et Innovation - UR UPJV 3900 (BIOPI), Université de Picardie Jules Verne (UPJV), Plateforme Analytique (PFA), Université de Picardie Jules Verne (UPJV)-Transfrontalière BioEcoAgro - UMR 1158 (BioEcoAgro), Université d'Artois (UA)-Université de Liège-Université de Picardie Jules Verne (UPJV)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Université d'Artois (UA)-Université de Liège-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL) |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Glycosylation [SDV]Life Sciences [q-bio] Proton Magnetic Resonance Spectroscopy Pharmaceutical Science Duboisia 01 natural sciences Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Glucosides Coumarins Scopoletin Drug Discovery Duboisia myoporoides Cells Cultured Pharmacology biology Organic Chemistry Tropane biology.organism_classification 030104 developmental biology Complementary and alternative medicine Biochemistry chemistry Proton NMR Molecular Medicine Scopolin Two-dimensional nuclear magnetic resonance spectroscopy 010606 plant biology & botany Tropanes |
| Zdroj: | Planta Medica Planta Medica, Georg Thieme Verlag, 2018, 84 (12/13), pp.971-975. ⟨10.1055/a-0632-2249⟩ Planta Medica, 2018, 84 (12/13), pp.971-975. ⟨10.1055/a-0632-2249⟩ |
| ISSN: | 1439-0221 0032-0943 |
| Popis: | Cell suspensions initiated from Duboisia myoporoides–a shrub belonging to the Solanaceae family and being a rich source of tropane alkaloids–previously showed their ability to glycosylate scopoletin into scopolin, which represent coumarins showing health benefits. To investigate the time course of this glycosylation reaction, an in vivo NMR approach was developed using a perfusion system in an 8-mm NMR tube and 1H NMR with 1D and 2D (TOCSY and NOESY) experiments. The time course of metabolic changes could therefore be followed without any labeling. |
| Databáze: | OpenAIRE |
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