Isolation and characterization of spontaneously arising auxotrophic and Kanagawa phenomenon-negative mutants of Vibrio parahaemolyticus
| Autor: | M J Voll, L A McNicol, D G Burstyn |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Vibriolysin
Nalidixic acid Auxotrophy Immunology Mutant Reversion Biology Arginine Microbiology Hemolysin Proteins Plasmid medicine Cysteine Amino Acids Gel electrophoresis Vibrio parahaemolyticus Purine Nucleosides biology.organism_classification Molecular biology Infectious Diseases Mutation Parasitology medicine.drug Plasmids Research Article |
| Zdroj: | Infection and immunity. 27(3) |
| ISSN: | 0019-9567 |
| Popis: | As a first step toward developing a system of genetic exchange between Vibrio parahaemolyticus strains, spontaneously arising auxotrophic and Kanagawa phenomenon-negative (KP-) mutants were isolated and characterized. Auxotrophic mutants were selected by nalidixic acid enrichment of parental cultures. Some Cys- and Arg- mutants of a KP+ strain were found to be KP-. Reversion to prototrophy by these strains was not accompanied by a return to the parental KP+ phenotype. Additionally, two prototrophic KP- mutants were isolated. No detectable levels of vibriolysin were found in supernatant extracts of KP- mutants by slide gel immunodiffusion analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, or assay for lethal activity in mice. All Cys-, Arg-, and Pur- mutants tested reverted to a different auxotrophy (phenotypic interconversion) as well as to prototrophy. The possible role of insertion sequence-like elements in vibriolysin production and phenotypic interconversion is discussed. |
| Databáze: | OpenAIRE |
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