Iron accumulation causes impaired myogenesis correlated with MAPK signaling pathway inhibition by oxidative stress
| Autor: | Keisuke Ishizawa, Licht Miyamoto, Toshiaki Tamaki, Masaki Imanishi, Tasuku Hirayama, Yuki Izawa-Ishizawa, Ken-ichi Aihara, Yoshito Zamami, Mizuki Imao, Yuya Horinouchi, Koichiro Tsuchiya, Yasumasa Ikeda, Akiho Satoh, Hiroaki Watanabe, Kenshi Takechi, Hideko Nagasawa, Hirofumi Hamano |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Cell Survival MAP Kinase Signaling System Iron Blotting Western muscle differentiation mitogen-activated protein kinases(MAPKs) Muscle Development medicine.disease_cause Thiobarbituric Acid Reactive Substances Biochemistry Cell Line Mice 03 medical and health sciences 0302 clinical medicine Cardiotoxin Genetics medicine Animals Myocyte RNA Messenger Muscle Skeletal Molecular Biology muscle regeneration ERK1/2 Hydroxyl Radical Chemistry Myogenesis Regeneration (biology) Skeletal muscle Cell biology Mice Inbred C57BL Oxidative Stress 030104 developmental biology medicine.anatomical_structure p38MAPK Phosphorylation myogenesis C2C12 030217 neurology & neurosurgery Oxidative stress Biotechnology |
| Zdroj: | The FASEB Journal. 33:9551-9564 |
| ISSN: | 1530-6860 0892-6638 |
| DOI: | 10.1096/fj.201802724rr |
| Popis: | Skeletal muscle atrophy is caused by disruption in the homeostatic balance of muscle degeneration and regeneration under various pathophysiological conditions. We have previously reported that iron accumulation induces skeletal muscle atrophy via a ubiquitin ligase-dependent pathway. However, the potential effect of iron accumulation on muscle regeneration remains unclear. To examine the effect of iron accumulation on myogenesis, we used a mouse model with cardiotoxin (CTX)-induced muscle regeneration in vivo and C2C12 mouse myoblast cells in vitro. In mice with iron overload, the skeletal muscles exhibited increased oxidative stress and decreased expression of satellite cell markers. Following CTX-induced muscle injury, these mice also displayed delayed muscle regeneration with a decrease in the size of regenerating myofibers, reduced expression of myoblast differentiation markers, and decreased phosphorylation of MAPK signaling pathways. In vitro, iron overload also suppressed the differentiation of C2C12 myoblast cells but the suppression could be reversed by superoxide scavenging using tempol. Excess iron inhibits myogenesis via oxidative stress, leading to an imbalance in skeletal muscle homeostasis.-Ikeda, Y., Satoh, A., Horinouchi, Y., Hamano, H., Watanabe, H., Imao, M., Imanishi, M., Zamami, Y., Takechi, K., Izawa-Ishizawa, Y., Miyamoto, L., Hirayama, T., Nagasawa, H., Ishizawa, K., Aihara, K.-I., Tsuchiya, K., Tamaki, T. Iron accumulation causes impaired myogenesis correlated with MAPK signaling pathway inhibition by oxidative stress. |
| Databáze: | OpenAIRE |
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