Cell-Laden Hydrogels in Integrated Microfluidic Devices for Long-Term Cell Culture and Tubulogenesis Assays
| Autor: | Marie Claude Hofmann, Nathan P. Gabrielson, Amit V. Desai, Bhushan Mahadik, Brendan A.C. Harley, Paul J. A. Kenis |
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| Rok vydání: | 2013 |
| Předmět: |
Tissue Engineering
Chemistry Microfluidics Cell Culture Techniques Biocompatible Materials Hydrogels General Chemistry Microfluidic Analytical Techniques Embryonic stem cell Cell biology Biomaterials Endothelial stem cell Tissue engineering Cell culture Self-healing hydrogels Animals Humans General Materials Science Stem cell Biochip Biotechnology |
| Zdroj: | Small. 9:3076-3081 |
| ISSN: | 1613-6810 |
| DOI: | 10.1002/smll.201203030 |
| Popis: | A hydrogel biochip combining microfluidic mixing and orthogonal supplementation strategies is developed and validated to allow facile generation of libraries of optically transparent 3D culture microenvironments. Live, on-chip tracing of embryonic stem cell differentiation and endothelial cell tubulogenesis confirms that the platform can be used to both create communities of discrete 3D microenvironments as well as to locally monitor subsequent divergent responses at both single cell and multi-cell scales. |
| Databáze: | OpenAIRE |
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