Transmission electron microscopy of small numbers of sorted cells
| Autor: | R. J. Sebring, N. F. Johnson, W. D. Spall |
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| Rok vydání: | 1988 |
| Předmět: |
Materials science
business.industry Biophysics Sorting Cell Separation Cell Biology Hematology Cell sorting Flow Cytometry Rats Pathology and Forensic Medicine Trachea Microscopy Electron Cell sorter chemistry.chemical_compound Endocrinology chemistry Transmission electron microscopy Animals Optoelectronics Agarose business Electron microscopic Sorted Cells |
| Zdroj: | Cytometry. 9:88-92 |
| ISSN: | 1097-0320 0196-4763 |
| Popis: | A new method that allows the transmission electron microscopic examination of as few as 1 x 10(4) cells obtained by flow cytometric sorting is described. The approach involves "sandwiching" fixed cells in an agarose case by a microcentrifugation system consisting of small-diameter cell-centrifugation tubes and subsequent processing of the cells by conventional techniques. The advantages offered by this method are discussed. |
| Databáze: | OpenAIRE |
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