Simultaneous inhibition of sulfate-reducing bacteria, removal of H2S and production of rhamnolipid by recombinant Pseudomonas stutzeri Rhl: Applications for microbial enhanced oil recovery
| Autor: | Jie Zhang, Siqin Han, Feng Zhao, Fang Ma, Ji-dong Zhou, Ying Zhang, Rongjiu Shi |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Bioaugmentation Environmental Engineering Bioengineering 010501 environmental sciences 01 natural sciences law.invention Microbiology chemistry.chemical_compound law 010608 biotechnology Oil and Gas Fields Hydrogen Sulfide Sulfate-reducing bacteria Waste Management and Disposal 0105 earth and related environmental sciences Pseudomonas stutzeri Recombination Genetic biology Strain (chemistry) Bacteria Renewable Energy Sustainability and the Environment Sulfates Rhamnolipid General Medicine Biodiversity biology.organism_classification Microbial enhanced oil recovery chemistry Recombinant DNA Glycolipids Oxidation-Reduction |
| Zdroj: | Bioresource technology. 207 |
| ISSN: | 1873-2976 |
| Popis: | Sulfate-reducing bacteria (SRB) are widely existed in oil production system, and its H2S product inhibits rhamnolipid producing bacteria. In-situ production of rhamnolipid is promising for microbial enhanced oil recovery. Inhibition of SRB, removal of H2S and production of rhamnolipid by recombinant Pseudomonas stutzeri Rhl were investigated. Strain Rhl can simultaneously remove S(2-) (>92%) and produce rhamnolipid (>136mg/l) under S(2-) stress below 33.3mg/l. Rhl reduced the SRB numbers from 10(9) to 10(5)cells/ml, and the production of H2S was delayed and decreased to below 2mg/l. Rhl also produced rhamnolipid and removed S(2-) under laboratory simulated oil reservoir conditions. High-throughput sequencing data demonstrated that addition of strain Rhl significantly changed the original microbial communities of oilfield production water and decreased the species and abundance of SRB. Bioaugmentation of strain Rhl in oilfield is promising for simultaneous control of SRB, removal of S(2-) and enhance oil recovery. |
| Databáze: | OpenAIRE |
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