Food from faeces: Evaluating the efficacy of scat DNA metabarcoding in dietary analyses
| Autor: | Kyle MacDonald, Femke Broekhuis, David Thuo, Dianne Gleeson, Joseph Kamau, Elise M. Furlan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Predation 01 natural sciences DNA barcoding Poultry Feces Acinonyx jubatus Gamefowl Mammals Meal Multidisciplinary biology Ecology Eukaryota Ruminants Animal Models Trophic Interactions Dna detection Community Ecology Experimental Organism Systems Vertebrates Leporids Medicine Rabbits Research Article Science Equines Wildlife Zoology Research and Analysis Methods 010603 evolutionary biology Birds 03 medical and health sciences Quails biology.animal DNA Barcoding Taxonomic Animals Life Science Horses Feeding ecology Cheetahs Deer Ecology and Environmental Sciences Organisms Correction Biology and Life Sciences DNA Diet 030104 developmental biology Fowl Amniotes Cats Animal Studies Acinonyx Chickens |
| Zdroj: | PLoS ONE 14 (2019) 12 PLoS ONE PLoS ONE, Vol 14, Iss 12, p e0225805 (2019) PLoS ONE, 14(12) |
| ISSN: | 1932-6203 |
| Popis: | Scat DNA metabarcoding is increasingly being used to track the feeding ecology of elusive wildlife species. This approach has greatly increased the resolution and detection success of prey items contained in scats when compared with other classical methods. However, there have been few studies that have systematically tested the applicability and reliability of this approach to study the diet of large felids species in the wild. Here we assessed the effectiveness of this approach in the cheetah Acinonyx jubatus. We tested how scat degradation, meal size, prey species consumed and feeding day (the day a particular prey was consumed) influenced prey DNA detection success in captive cheetahs. We demonstrated that it is possible to obtain diet information from 60-day old scats using genetic approaches, but the efficiency decreased over time. Probability of species-identification was highest for food items consumed one day prior to scat collection and the probability of being able to identify the species consumed increased with the proportion of the prey consumed. Detection success varied among prey species but not by individual cheetah. Identification of prey species using DNA detection methods from a single consumption event worked for samples collected between 8 and 72 hours post-feeding. Our approach confirms the utility of genetic approaches to identify prey species in scats and highlight the need to account for the systematic bias in results to control for possible scat degradation, feeding day, meal size and prey species consumed especially in the wild-collected scats. |
| Databáze: | OpenAIRE |
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